Nanopore-based Fourth-generation DNA Sequencing Technology

• Published in: Genomics, proteomics & bioinformatics Vol. 13; no. 1; pp. 4 - 16
• Main Authors: Feng, Yanxiao, Zhang, Yuechuan, Ying, Cuifeng, Wang, Deqiang, Du, Chunlei
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.02.2015; Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; Elsevier; Oxford University Press
• Subjects: academic achievement; DNA; DNA sequencing; DNA测序技术; genome; Genome, Human; Humans; molecular biology; Nanopore; Nanopores; Nanotechnology - instrumentation; Nanotechnology - methods; Review; sequence analysis; Sequence Analysis, DNA - instrumentation; Sequence Analysis, DNA - methods; Single base; Single molecule; 分子尺度; 分子生物学; 单分子技术; 相互作用; 第四代; 纳米孔; 蛋白质; Single base; Nanopore; DNA sequencing; Single molecule
• ISSN: 1672-0229; 2210-3244
• DOI: 10.1016/j.gpb.2015.01.009

Nanopore-based sequencers, as the fourth-generation DNA sequencing technology, have the potential to quickly and reliably sequence the entire human genome for less than $1000, and possibly for even less than $100. The single-molecule techniques used by this technology allow us to further study the interaction between DNA and protein, as well as between protein and protein.Nanopore analysis opens a new door to molecular biology investigation at the single-molecule scale.In this article, we have reviewed academic achievements in nanopore technology from the past as well as the latest advances, including both biological and solid-state nanopores, and discussed their recent and potential applications.