Augmentation of Interferon Production after Cell-Differentiation of U937 Cells by TPA

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 40; no. 9; pp. 675 - 679
• Main Authors: Indoh, Tomokazu, Shirakawa, Sachiko, Kubota, Tohru, Yashiki, Teruo, Isogai, Emiko, Fujii, Nobuhiro
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01.01.1996; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: 2',5'-Oligoadenylate synthetase; 2',5'-Oligoadenylate Synthetase - metabolism; 5′-oligoadenylate synthetase; Biological and medical sciences; Cell Differentiation - drug effects; Cells, Cultured; Fundamental and applied biological sciences. Psychology; Humans; IFN; Interferons - biosynthesis; Interferons - pharmacology; Microbiology; Monocytes; Mumps - immunology; Mumps - virology; Mumps virus; Mumps virus - growth & development; Persistent infection; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; RNA, Messenger - biosynthesis; T-Lymphocytes; Tetradecanoylphorbol Acetate - pharmacology; TPA; U937; U937, 2′,5′‐oligoadenylate synthetase; Virology; Human; Histiocytic lymphoma; Enzyme; Cytokine; Cell differentiation; Host virus relation; Paramyxoviridae; Virus; Cell line; Enzymatic activity; Persistent infection; Interferon; Infected cell; Mumps paramyxovirus; Paramyxovirus
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1996.tb01127.x

Persistent infections with mumps virus were established in several human lymphoid cells of T-cell origin (Molt-4, TALL-1, and CCRF-CEM) and human monocyte cells (U937 and THP-1). 2', 5'-Oligoadenylate synthetase (2-5AS) activity was demonstrated to be only slightly induced by interferon (IFN) or TPA (12-O-tetradecanoyl-phorbol-13-acetate) treatment in these cells. Treatment of the persistently infected cells with IFN or TPA did not stimulate an increase in the amount of synthetase mRNA. Induction of cell differentiation and augmentation of IFN production by TPA were demonstrated in U937 cells persistently infected with mumps virus (U937-MP). Similar results for IFN production were obtained from differentiated U937 cells. It is suggested that cell differentiation of U937 cells might be associated with the development of IFN inducibility.