Resistance of Pseudomonas pseudomallei to Normal Human Serum Bactericidal Action

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 32; no. 7; pp. 645 - 652
• Main Authors: Ismail, Ghazally, Razak, Nyonya, Mohamed, Rahmah, Embi, Noor, Omar, Othman
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01.01.1988; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: Action of physical and chemical agents on bacteria; Adult; Bacteria; Bacteriology; Biological and medical sciences; Blood Bactericidal Activity; Fundamental and applied biological sciences. Psychology; Humans; In Vitro Techniques; Male; Melioidosis - blood; Melioidosis - immunology; Microbiology; Pseudomonas - immunology; Pseudomonas pseudomallei; Sepsis - blood; Sepsis - immunology; Pseudomonadales; Human; Pseudomonas pseudomallei; Sensitivity resistance; Bacteria; Pseudomonadaceae; Serum; Bactericidal effect
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1988.tb01426.x

The effect of human normal serum (HNS) on Pseudomonas pseudomallei was determined. It is apparent from our data that the organism is resistant to the normal serum bactericidal mechanism. Ancillary experiments to confirm this serum-resistant property of P. pseudomallei were done by examining the effects of growth phase conditions of the bacteria (i.e., logarithmic and stationary phases) and different buffered systems used as diluent in our bactericidal assay. Results obtained showed similar degree of resistance to serum bactericidal killing by 5 strains of the organisms tested. The possible survival advantage of serum-resistant property to P. pseudomallei as bacterial pathogens known to invade the blood stream is discussed.