Biological Activities of Lipopolysaccharide-Like Substance (LLS) Extracted from Leptospira interrogans Serovar canicola Strain Moulton

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 31; no. 8; pp. 727 - 735
• Main Authors: Shimizu, Tadayori, Matsusaka, Eiichiro, Takayanagi, Kazuhisa, Masuzawa, Toshiyuki, Iwamoto, Yoshihisa, Morita, Tamotsu, Mifuchi, Ichiji, Yanagihara, Yasutake
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01.01.1987; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: Adjuvants, Immunologic - pharmacology; Animals; Antineoplastic Agents - pharmacology; B-Lymphocytes - drug effects; Bacteriology; Biological and medical sciences; Cells, Cultured; Female; Fundamental and applied biological sciences. Psychology; Leptospira interrogans; Lipopolysaccharides - immunology; Lipopolysaccharides - pharmacology; Lipopolysaccharides - toxicity; Lymphocyte Activation - drug effects; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microbiology; Mitogens - pharmacology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Salmonella typhimurium; T-Lymphocytes - drug effects; Antineoplastic agent; Cell culture; Spirochaetales; Toxicity; Mortality; Rodentia; B-Lymphocyte; Splenocyte; Experimental animal; Biological activity; Vertebrata; Experimental disease; Mammalia; Mouse; Immunological adjuvant; Leptospiraceae; Lipopolysaccharide; Bacteria; Mitogen; Leptospira interrogans serovar canicola; T»-Lymphocyte; Comparative study; Gram negative bacteria
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1987.tb03134.x

The biological activities of lipopolysaccharide-like substance (LLS) extracted from Leptospira interrogans serovar canicola strain Moulton by the hot phenol-water method were studied in mice. The addition of 12.5μg/ml or more of LLS fraction increased the incorporation of [3H]thymidine into in vitro cultured spleen cells of C57BL/6 mice, while the activity of the LLS fraction was about 20 times weaker than that of Salmonella typhimurium lipopolysaccharide (LPS). Pretreatment of murine spleen cells with rabbit anti-mouse thymocyte antiserum did not diminish the mitogenic activity of leptospiral LLS, and the LLS could not increase the incorporation of [3H]thymidine into thymocytes, suggesting that LLS acts on a B-lymphocyte population of lymphocytes. When sheeperythrocytes and LLS fraction were injected intraperitoneally into BALB/c mice, LLS exhibited an enhancing effect on antibody response in vivo. However, lethal toxicity of the LLS fraction was about 500 times lower than that of LPS in C57BL/6 mice loaded with galactosamine. No antitumor activity of leptospiral LLS (250-1, 000μg/mouse) against the ascites form of Ehrlich carcinoma in ddY mice was observed. The biological activities of the LLS fraction from the organism were weaker than those of gram-negative bacterial LPS, suggesting that Leptospira possesses no typical LPS.