Sheep Red Blood Cell Instillation at Palatine Tonsil Effectively Induces Specific IgA Class Antibody in Saliva in Rabbits

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 39; no. 5; pp. 351 - 359
• Main Authors: Fukuizumi, Takaki, Inoue, Hiromasa, Anzai, Yuichi, Tsujisawa, Toshiyuki, Uchiyama, Choji
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01.01.1995; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: Administration, Intranasal; Analysis of the immune response. Humoral and cellular immunity; Animals; Antibody production; Antibody-Producing Cells - immunology; Antigens - administration & dosage; Biological and medical sciences; Chemotaxis, Leukocyte; Erythrocytes - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Immunoglobulin A - biosynthesis; Lymphocyte Count; Lymphoid Tissue - immunology; Mucosal immunity; Organ Specificity; Palatine tonsil; Palatine Tonsil - immunology; Rabbits; Saliva; Saliva - immunology; Salivary Proteins and Peptides - immunology; Secretory IgA; Sheep - blood; Stomach; Oral cavity; Vertebrata; IgA; Local immunity; Mammalia; Immune response; Mouse; Antibody; Rodentia; Saliva; Humoral immunity
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1995.tb02212.x

We have shown that the palatine tonsil effectively incorporates exogenous foreign substances instilled at its surface. It is not clear whether antigen-specific IgA can be induced by the instillation. Sheep red blood cells (SRBC) were instilled at the palatine tonsil every three days as the antigen, and the agglutination titer of specific IgA in saliva was examined. Nasal or intragastric administration, which have been shown to induce specific antibody in saliva, were done as control experiments. Anti-SRBC antibody in saliva from the tonsillar instillation group was detected in the second week, and the agglutination titer reached a maximum in the 6th week after the instillation. The maximum titers in the tonsillar instillation group and nasal administration group were 16 (P<0.01, n=7) and 4 times (P<0.01, n=7) higher, respectively, than that in the intragastric administration group. In the tonsillar instillation group, the number of specific antibody-producing cells per 105 lymphocytes was the highest in the parotid glands compared with the lymphoid tissues such as the retropharyngeal lymph nodes, nasal mucosa, mesenteric lymph nodes, Peyer's patches, cervical lymph nodes, palatine tonsil and spleen. In the nasal administration group, the number of lymphocytes was the highest in the nasal mucosa. The results indicate that tonsillar instillation was more effective than nasal administration in inducing specific IgA in saliva.