Preliminary Phytochemical Screening and Antibacterial Activity of Leaves ofPassiflora foetida Linn

• Published in: RGUHS journal of pharmaceutical sciences Vol. 12; no. 2
• Main Authors: E, Akila, Haroon, Mohammed
• Format: Journal Article
• Language: English
• Published: 2022
• ISSN: 2249-2208
• DOI: 10.26463/rjps.12_2_5

Background Passiflora foetida is widely used in Indian traditional system of medicines to cure various human diseases.Aim The present study envisaged to select an appropriate local plant with anti-bacterial ability based on its folklore claim and to scientifically establish its anti-bacterial activity.Methods Shade dried powdered material of leaves of Passiflora foetida Linn. was used for the determination of physiochemical constants in accordance with WHO guidelines. Subsequent solvent extracts were prepared using petroleum ether chloroform ethanol and water. Each extract was further proceeded for detailed phytochemical study and anti-bacterial screening.Results The preliminary phytochemical screening revealed the chemical constituents of alkaloids flavonoids and phenolic compounds. In vitro antibacterial studies on the leaf extracts were carried out on medically important micro-organisms such as Escherichia coli Staphylococcus aureus Klebsiella pneumoniae Staphylococcus epidermis and Pseudomonas aeruginosa. Growth of all cultures was seen in the solvent control and in positive control. Petroleum ether and chloroform extracts showed inhibition of growth of microorganisms like Escherichia coli Staphylococcus aureus Klebsiella pneumoniae and Staphylococcus epidermis at 200 microgmL. No growth was seen for Pseudomonas aeruginosa at the concentration of 300 microgmL. Ethanol and aqueous extracts exhibited inhibitory effect on the growth of Escherichia coli Staphylococcus aureus Klebsiella pneumoniae and Staphylococcus epidermis at 200 microgmL and Pseudomonas aeruginosa at the concentration of 100 microgmL. A dose dependent inhibitory activity was observed.Conclusion From the above findings it was clear that the secondary metabolites present in leaf extracts of Passiflora foetida Linn are the source of plant-derived antimicrobial substances.