Cloning and Sequence Analysis of Transferrin Binding Protein Gene A of Field and Vaccine Strains of Pasteurella multocida B:2

• Published in: Indian journal of animal research no. Of
• Main Authors: Sharma, Himanshu, Grover, Y.P., Singh, Mahavir, Mishra, Richa, Kumar, Pankaj, ., Ojasvita, Kumar, Arvind, Singh, Ajit, Diwakar, R.P.
• Format: Journal Article
• Language: English
• Published: 11.09.2021
• ISSN: 0367-6722; 0976-0555
• DOI: 10.18805/IJAR.B-4440

Background: Pasteurella multocida is an important bacterial pathogen that causes many major diseases of which haemorrhagic septiciemia (HS) in cattle and buffalo is responsible for catastrophic epizootics in India and South Asia. In India, the disease haemorrhagic septiciemia is considered as the most dreaded bacterial disease. Various host- and pathogen- specific determinants are responsible for disease outcome. Various bacterial virulence genes (tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 and ptfA) have been proposed to play a key role in this interaction. Methods: The present study was done to compare the gene and deduced amino acid sequence of transferrin binding protein gene (tbpA) gene of field isolates and vaccine strain of P. multocida B: 2. Result: It was observed that tbpA gene of field and vaccine strains have similar nucleotide sequence except at positions 574 and 620. The sequence of tbpA gene was used for prediction of matured TbpA protein characteristics. The deduced amino acid sequences of 242 amino acids revealed 99% homology with TbpA of P. multocida and with a variety of other TonB-dependent receptor proteins, indicating that it belongs to the family of outer membrane receptors. Deduced amino acid sequence was found to be similar in field and vaccine strains except at 207th amino acid. In field isolates Leucine was there while in vaccine strain Phenyl alanine was found. These both amino acids are hydrophobic in nature so no change in physico-chemical property of TbpA is expected. From this study it is concluded that single amino acid difference between field isolate and vaccine strain might not cause change in its binding and physico-chemical property.