Differentially Expressed Proteins Associated with Fusarium Head Blight Resistance in Wheat

• Published in: PloS one Vol. 8; no. 12; p. e82079
• Main Authors: Zhang, Xianghui, Fu, Jianming, Hiromasa, Yasuaki, Pan, Hongyu, Bai, Guihua, Yan, Wengui
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.12.2013; Public Library of Science (PLoS)
• Subjects: Agriculture; alleles; Antifungal agents; Arabidopsis; Blight; Comparative analysis; Crop yield; Cultivars; Dehydrogenases; Detoxification; Disease Resistance - genetics; Disease Resistance - immunology; Electrophoresis, Polyacrylamide Gel; Energy metabolism; Fungi; Fusarium; Fusarium - physiology; Fusarium graminearum; Fusarium head blight; Gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant; Genomes; Grain; grain yield; Health aspects; Infection; Infections; Inoculation; Kinases; Medical research; Metabolism; Mitochondrial DNA; Mycoses; Photosynthesis; Physiological aspects; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; protein synthesis; Proteins; Proteomics; Spectrometry, Mass, Electrospray Ionization; Studies; Triticum - genetics; Triticum - immunology; Triticum - microbiology; Wheat; wheat protein; United States > US; China; Kansas
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0082079

Fusarium head blight (FHB), mainly caused by Fusarium graminearum , substantially reduces wheat grain yield and quality worldwide. Proteins play important roles in defense against the fungal infection. This study characterized differentially expressed proteins between near-isogenic lines (NILs) contrasting in alleles of Fhb1 , a major FHB resistance gene in wheat, to identify proteins underlining FHB resistance of Fhb1 . The two-dimensional protein profiles were compared between the Fusarium -inoculated spikes of the two NILs collected 72 h after inoculation. The protein profiles of mock- and Fusarium -inoculated Fhb1 + NIL were also compared to identify pathogen-responsive proteins. Eight proteins were either induced or upregulated in inoculated Fhb1 + NIL when compared with mock-inoculated Fhb1 + NIL; nine proteins were either induced or upregulated in the Fusarium -inoculated Fhb1 + NIL when compared with Fusarium -inoculated Fhb1 − NIL. Proteins that were differentially expressed in the Fhb1 + NIL, not in the Fhb1 − NIL, after Fusarium inoculation included wheat proteins for defending fungal penetration, photosynthesis, energy metabolism, and detoxification. Coordinated expression of the identified proteins resulted in FHB resistance in Fhb1 + NIL. The results provide insight into the pathway of Fhb1 -mediated FHB resistance.