Comparative evaluation of two ELISA kits for detecting antibodies to a nonstructural protein of foot-and-mouth disease virus using serum samples collected from naturally and experimentally infected cows

• Published in: Journal of Veterinary Medical Science Vol. 80; no. 10; pp. 1624 - 1630
• Main Authors: FUKAI, Katsuhiko, NISHI, Tatsuya, SHIMADA, Nobuaki, MORIOKA, Kazuki, YAMADA, Manabu, YOSHIDA, Kazuo, YAMAKAWA, Makoto
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2018; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Antibodies; Antibodies, Viral - blood; Cattle; Cattle Diseases - immunology; Cattle Diseases - virology; ELISA; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Female; Foot & mouth disease; Foot-and-Mouth Disease - immunology; Foot-and-mouth disease virus; Foot-and-Mouth Disease Virus - immunology; Immunoglobulins; Japan; nonstructural protein; Sensitivity and Specificity; Vaccination; Viral Nonstructural Proteins - immunology; Viral Vaccines - administration & dosage; Viral Vaccines - immunology; Virology; Japan; Foot-and-mouth disease virus; cattle; ELISA; nonstructural protein
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.17-0662

When foot-and-mouth disease (FMD) occurs and a “vaccination-to-live” policy is adopted in a country, the country must perform serological surveillance of a nonstructural protein (NSP) of FMD virus. The NCPanaftosa kit is the only kit for detecting antibodies to NSPs that is officially recognized as the reference regent by the World Organization for Animal Health; however, it is only used in South American countries. In this study, the specificity and sensitivity of the NCPanaftosa kit were compared with those of the PrioCHECK kit sold by an international company. Results in this study suggest that the PrioCHECK kit performs similarly to the NCPanaftosa kit in detecting antibodies to the NSP in the cattle population.