Multiplex PCR ( mPCR ) for the Detection of S almonella spp. and the Differentiation of the T yphimurium and E nteritidis Serovars in Chicken Meat
Abstract S almonella enterica subspecies enterica serovars Enteritidis and Typhimurium are important causes of foodborne illness. Methods for simultaneous detection of these serovars may contribute for the adoption of measures to prevent these diseases. The polymerase chain reaction to detect indivi...
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Published in | Journal of food safety Vol. 33; no. 1; pp. 25 - 29 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.02.2013
|
Online Access | Get full text |
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Summary: | Abstract
S
almonella enterica
subspecies
enterica
serovars Enteritidis and Typhimurium are important causes of foodborne illness. Methods for simultaneous detection of these serovars may contribute for the adoption of measures to prevent these diseases. The polymerase chain reaction to detect individually or simultaneously the serovars Enteritidis and Typhimurium in foods have been standardized; however, the majority of assays employ the
fli
C
gene as a target for detection of serovar Typhimurium. The detection of these sorovars in a few hours allows the food supply chain to take appropriate measures to prevent the distribution of contaminated food. The aim of this study was to develop a new multiplex
PCR
(
mPCR
) for the simultaneous detection and differentiation of
S
almonella
spp.,
S
.
Enteritidis and
S
.
Typhimurium in chicken meat. The
mPCR
assays showed high specificity and differentiated
S
.
Typhimurium from 22
S
almonella
serovars tested, including
S
.
K
entucky, showing that it's an alternative to reduce the time required to obtain presumptive positive results.
Practical Applications
The
fli
C
gene used as target for the detection of serovar
T
yphimurium is questionable as it has also been described for
S
.
K
entucky. The amplification of
STM
4492 gene in this study, instead of
fli
C
gene, exhibited high specificity to detect
S
.
Typhimurium. The developed
mPCR
is an efficient means for the simultaneous detection and differentiation of
S
almonella
spp.,
S
.
Enteritidis and
S
.
Typhimurium in chicken meat after 24 h of enrichment. The
mPCR
has the potential to be used in routine diagnostic laboratories to obtain presumptive positive results and for identification of
E
nteritidis and
T
yphimurium strains isolated by the conventional method. |
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ISSN: | 0149-6085 1745-4565 |
DOI: | 10.1111/jfs.12019 |