RBM 28, a protein deficient in ANE syndrome, regulates hair follicle growth via miR‐203 and p63
Abstract Alopecia–neurological defects–endocrinopathy ( ANE ) syndrome is a rare inherited hair disorder, which was shown to result from decreased expression of the RNA ‐binding motif protein 28 ( RBM 28 ). In this study, we attempted to delineate the role of RBM 28 in hair biology. First, we sought...
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Published in | Experimental dermatology Vol. 24; no. 8; pp. 618 - 622 |
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Main Authors | , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.08.2015
|
Online Access | Get full text |
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Summary: | Abstract
Alopecia–neurological defects–endocrinopathy (
ANE
) syndrome is a rare inherited hair disorder, which was shown to result from decreased expression of the
RNA
‐binding motif protein 28 (
RBM
28
). In this study, we attempted to delineate the role of
RBM
28 in hair biology. First, we sought to obtain evidence for the direct involvement of
RBM
28 in hair growth. When
RBM
28 was downregulated in human hair follicle (
HF
) organ cultures, we observed catagen induction and
HF
growth arrest, indicating that
RBM
28 is necessary for normal hair growth. We also aimed at identifying molecular targets of
RBM
28. Given that an
RBM
28 homologue was recently found to regulate mi
RNA
biogenesis in
C. elegans
and given the known pivotal importance of mi
RNA
s for proper hair follicle development, we studied global mi
RNA
expression profile in cells knocked down for
RBM
28. This analysis revealed that
RBM
28 controls the expression of miR‐203. miR‐203 was found to regulate in turn
TP
63
, encoding the transcription factor p63, which is critical for hair morphogenesis. In conclusion,
RBM
28 contributes to
HF
growth regulation through modulation of miR‐203 and p63 activity. |
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ISSN: | 0906-6705 1600-0625 |
DOI: | 10.1111/exd.12737 |