Involvement of PAPP ‐A and IGFR 1 in Cystic Ovarian Disease in Cattle
Contents Cystic ovarian disease ( COD ) is one of the main causes of infertility in dairy cattle. It has been shown that intra‐ovarian factors, such as members of the insulin‐like growth factor ( IGF ) system, may contribute to follicular persistence. The bioavailability of IGF to initiate its respo...
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Published in | Reproduction in domestic animals Vol. 50; no. 4; pp. 659 - 668 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.08.2015
|
Online Access | Get full text |
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Summary: | Contents
Cystic ovarian disease (
COD
) is one of the main causes of infertility in dairy cattle. It has been shown that intra‐ovarian factors, such as members of the insulin‐like growth factor (
IGF
) system, may contribute to follicular persistence. The bioavailability of
IGF
to initiate its response by binding to specific receptors (
IGFR
s) depends on interactions with related compounds, such as pregnancy‐associated plasma protein A (
PAPP
‐A). The aim of this study was to determine
IGFR
1 and
PAPP
‐A expression both in follicles at different stages of development and in cysts, to evaluate the roles in the etiopathogenesis of
COD
in cattle. The
mRNA
expression of
PAPP
‐A was higher in granulosa cells of large tertiary follicles than in cysts, whereas the protein
PAPP
‐A present in the follicular fluid from these follicles showed no differences. Although no
PAPP
‐A
mRNA
expression was detected in smaller tertiary follicles, in their follicular fluid, this protease was detected in lesser concentration than in cysts. The
mRNA
expression of
IGFR
1 was lower in granulosa cells from cystic follicles than in those from tertiary ones. However, the protein expression of this receptor presented the highest levels in cystic structures, probably to increase the possibility of
IGF
response. The data obtained would indicate that animals with
COD
have an altered regulation of the
IGF
system in the ovary, which could be involved in the pathogenesis of this disease in cattle. |
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ISSN: | 0936-6768 1439-0531 |
DOI: | 10.1111/rda.12547 |