A Comparative Fluorescent Beacon-based Method for Serum microRNA Quantification

Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucle...

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Bibliographic Details
Published inAnalytical Sciences Vol. 31; no. 3; pp. 231 - 235
Main Authors BETA, Madhu, KRISHNAKUMAR, Subramanian, ELCHURI, Sailaja V, SALIM, Bindu, NARAYANAN, Janakiraman
Format Journal Article
LanguageEnglish
Published Singapore The Japan Society for Analytical Chemistry 2015
Springer Nature Singapore
Japan Science and Technology Agency
Subjects
Analytical Chemistry
Biomarkers
Chemistry
Circulating
Fluorescence
Fluorescent Dyes - chemistry
Human
Humans
Locked nucleic acid (LNA)
Mathematical analysis
MicroRNAs - blood
MicroRNAs - chemistry
Microscopy
Microscopy, Fluorescence
miRNA
Nucleic Acid Hybridization
Oligonucleotide Probes - chemistry
Oligonucleotides - chemistry
retinoblastoma
Retinoblastoma - blood
Ribonucleic acids
Serum
Serums
Software
Spectrometry, Fluorescence
Locked nucleic acid (LNA)
miRNA
serum
retinoblastoma
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Summary:Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 μL healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.31.231