The oligodendrocyte‐specific antibody ‘ CC 1’ binds Quaking 7

Abstract The mouse monoclonal antibody marketed as anti‐adenomatous polyposis coli clone CC 1, often referred to as CC 1, is the antibody most commonly used to specifically label mature oligodendrocytes without labeling myelin. Previous studies have shown that despite being raised against adenomatou...

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Bibliographic Details
Published inJournal of neurochemistry Vol. 139; no. 2; pp. 181 - 186
Main Authors Bin, Jenea M., Harris, Stephanie N., Kennedy, Timothy E.
Format Journal Article
LanguageEnglish
Published 01.10.2016
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Summary:Abstract The mouse monoclonal antibody marketed as anti‐adenomatous polyposis coli clone CC 1, often referred to as CC 1, is the antibody most commonly used to specifically label mature oligodendrocytes without labeling myelin. Previous studies have shown that despite being raised against adenomatous polyposis coli, this antibody binds another unknown antigen. We show that the CC 1 antibody binds Quaking 7, an RNA ‐binding protein that is highly up‐regulated in myelinating oligodendrocytes in the central nervous system. image The monoclonal antibody anti‐adenomatous polyposis coli (APC) clone CC1, is the antibody most commonly used to specifically label the cell bodies of mature oligodendrocytes. Despite being raised against APC, previous studies showed this antibody binds another unknown antigen. We show that the CC1 antibody binds Quaking (QKI) 7, an RNA‐binding protein which is highly up‐regulated in myelinating oligodendrocytes.
ISSN:0022-3042
1471-4159
DOI:10.1111/jnc.13745