Novel Biological Activity of the Region (106—126) on Human Prion Sequence

We report that the synthetic peptide Prp106—126 (KTNMKHMAGAAAAGAVVGGLG-COOH) and the reversed peptide Prp126—106 (GLGGVVAGAAAAGAMHKMNTK-COOH) of human prion (hPrp) can express the decarboxylase activity for oxaloacetate in the presence of trifluoroethanol, similar to that of Oxaldie 1 (LAKLLKALAKLLK...

Full description

Saved in:
Bibliographic Details
Published inBiological & Pharmaceutical Bulletin Vol. 26; no. 2; pp. 229 - 232
Main Authors Numao, Naganori, Noguchi, Norihisa, Eguchi, Yukihiro, Watanabe, Satoshi, Fukui, Tetsuya, Kamino, Tomoyuki, Shimozono, Noriko, Yamazaki, Akiko, Kobayashi, Susumu, Sasatsu, Masanori
Format Journal Article
LanguageEnglish
Japanese
Published Tokyo The Pharmaceutical Society of Japan 01.02.2003
Pharmaceutical Society of Japan
Maruzen
Japan Science and Technology Agency
Subjects
Amino Acid Sequence - drug effects
Amino Acid Sequence - physiology
bioinformatics
Biological and medical sciences
Carboxy-Lyases - metabolism
decarboxylation
Enzyme Activation - drug effects
Enzyme Activation - physiology
Humans
Medical sciences
Molecular Sequence Data
oxaloacetate
Peptide Fragments - genetics
Peptide Fragments - pharmacokinetics
Peptide Fragments - pharmacology
prion
Prions - genetics
Prions - pharmacokinetics
Prions - pharmacology
synthetic peptide
prion
decarboxylation
bioinformatics
oxaloacetate
synthetic peptide
Online AccessGet full text

Cover

More Information
Summary:We report that the synthetic peptide Prp106—126 (KTNMKHMAGAAAAGAVVGGLG-COOH) and the reversed peptide Prp126—106 (GLGGVVAGAAAAGAMHKMNTK-COOH) of human prion (hPrp) can express the decarboxylase activity for oxaloacetate in the presence of trifluoroethanol, similar to that of Oxaldie 1 (LAKLLKALAKLLKK-CONH2) reported previously. The degree of the relative activity of Prp106—126 and Prp126—106 to Oxaldie 1 is 0.47 and 0.21, respectively. Based on this experimental result, we applied the informational system method (ISM) developed by Veljkovic et al. to the amino acid sequence of Prp106—126 and Prp126—106 to extract a common factor. The same spectra were obtained, indicating that the same periodicity may be conserved on their sequences, as a necessary factor for expressing the same biological activity, irrespective of the orientation of the primary sequence.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.26.229