pir A‐ and pirB ‐like gene identification in Micrococcus luteus strains in Mexico
Abstract Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009 and afterwards in Mexico in 2013. AHPND is caused by Vibrio parahaemolyticus and affects Penaeus monodon and Litopenaeus vannamei shrimp cultures. The bacterium contains the pirA‐ and pirB‐ like genes in 69‐...
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Published in | Journal of fish diseases Vol. 41; no. 11; pp. 1667 - 1673 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.11.2018
|
Online Access | Get full text |
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Summary: | Abstract
Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009 and afterwards in Mexico in 2013. AHPND is caused by
Vibrio parahaemolyticus
and affects
Penaeus monodon
and
Litopenaeus vannamei
shrimp cultures. The bacterium contains the
pirA‐
and
pirB‐
like genes in 69‐ to 70‐Kb plasmids, which encode the toxins that produce the disease. The aim of this study was to determine whether
pirA‐
and
pirB
‐like genes existed in bacterial genera distinct from
Vibrio
before the first cases of AHPND were documented in Mexico. Two bacterial isolates were selected from shrimp farms in Nayarit in 2006 and analysed by nested‐PCR to determine the presence of
pirA
‐ and
pirB
‐like genes. The two isolates chosen did indeed show the presence of these genes, and those findings were confirmed by sequencing. Both strains matched to the bacterial species
Micrococcus luteus
. Results revealed two important situations: (a) the
pirA
‐ and
pirB
‐like genes were present in a bacterial species that has not been reported previously (
Micrococcus luteus
); and (b)
pirA
‐ and
pirB
‐like bacterial genes were present in Mexico before the first AHPND outbreak was reported in China. |
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ISSN: | 0140-7775 1365-2761 |
DOI: | 10.1111/jfd.12874 |