Induction of Heme Oxygenase-1 Inhibits Monocyte Chemoattractant Protein-1 mRNA Expression in U937 Cells

• Published in: Journal of Pharmacological Sciences Vol. 100; no. 2; pp. 162 - 166
• Main Authors: Shokawa, Tomoki, Yoshizumi, Masao, Yamamoto, Hideya, Omura, Shinji, Toyofuku, Mamoru, Shimizu, Yoshito, Imazu, Michinori, Kohno, Nobuoki
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 2006; The Japanese Pharmacological Society; Elsevier
• Subjects: Chemokine CCL2 - antagonists & inhibitors; Chemokine CCL2 - genetics; Dose-Response Relationship, Drug; Enzyme Induction; Gene Expression Regulation - drug effects; heme oxygenase-1; Heme Oxygenase-1 - biosynthesis; Heme Oxygenase-1 - genetics; Humans; Lysophosphatidylcholines - pharmacology; monocyte chemoattractant protein-1; Monocytes - cytology; Monocytes - drug effects; oxidized low-density lipoprotein; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; RNA, Ribosomal, 18S - metabolism; Time Factors; U937 Cells; monocyte chemoattractant protein-1; oxidized low-density lipoprotein; heme oxygenase-1
• ISSN: 1347-8613; 1347-8648
• DOI: 10.1254/jphs.SC0040188

Heme oxygenase-1 (HO-1) is a stress-inducible isoform of HO with potential cytoprotective effects. Monocyte activation/migration mediated by monocyte chemoattractant protein-1 (MCP-1) is one of the earliest and important events in the pathogenesis of atherosclerosis. We examined the effect of HO-1 on the production of lysophosphatidylcholine (Lyso-PC)-induced MCP-1 in the human promonocytic cell line U937. Increased HO-1 induction by hemin resulted in a significant decrease in the Lyso-PC-mediated induction of MCP-1 mRNA expression. SnPP (IX), the specific inhibitor of HO-1 enzymatic activity, prevented the hemin-mediated attenuation of MCP-1 mRNA expression. These results suggest that HO-1 may work as an anti-atherogenic agent through the attenuation of MCP-1 production.