When genes go wild: highly variable internal transcibed spacer 1 and conserved mitochondrial DNA haplotypes used to examine the genetic diversity and dispersal pathways of invasive Hylotrupes bajulus in W estern A ustralia
Abstract Hylotrupes bajulus ( L innaeus) is a cryptic insect pest of seasoned coniferous timber. In 2004, an incursion of Hylotrupes bajulus was discovered in P erth, W estern A ustralia. Two genes, one mitochondrial ( cytochrome oxidase subunit I ; COI ) and one nuclear ( i nternal t ranscribed s...
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Published in | Agricultural and forest entomology Vol. 15; no. 3; pp. 236 - 244 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.08.2013
|
Online Access | Get full text |
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Summary: | Abstract
Hylotrupes bajulus
(
L
innaeus) is a cryptic insect pest of seasoned coniferous timber. In 2004, an incursion of
Hylotrupes bajulus
was discovered in
P
erth,
W
estern
A
ustralia.
Two genes, one mitochondrial (
cytochrome oxidase
subunit
I
;
COI
) and one nuclear (
i
nternal
t
ranscribed
s
pacer 1;
ITS1
) were used to infer the origin of this incursion, as well as to test the likely dispersal pathways and dispersal distances of
H. bajulus
.
We sequenced and examined 1003 cloned
ITS1
fragments and 170
COI
fragments from 14 international and 156 domestic specimens. The
ITS1
fragment was extremely variable, with 94% of the sites polymorphic.
To remove the variability and select only the informative polymorphisms principal component analysis was employed. This resulted in the selection of eight informative single nucleotide polymorphisms and seven microsatellites, which were converted into 119 unique
ITS
haplotypes. In comparison,
COI
was highly conserved, yielding only four haplotypes.
The data suggest at least three independent incursions, of which two contained the same mitochondrial haplotype but different
ITS1
sequences. The datasets were further scrutinized to estimate local dispersal distances. The results suggested that individuals were capable of dispersing > 14 km, which is significantly further than previously reported. |
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ISSN: | 1461-9555 1461-9563 |
DOI: | 10.1111/afe.12010 |