Retracted: 14‐3‐3ζ promotes gliomas cells invasion by regulating Snail through the PI 3K/ AKT signaling
Abstract 14‐3‐3ζ has been reported to function as critical regulators of diverse cellular responses. However, the role of 14‐3‐3ζ in gliomas progression remains largely unknown. The expression level of 14‐3‐3ζ and Snail was detected by Western blot analysis and quantitative polymerase chain reaction...
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Published in | Cancer medicine (Malden, MA) Vol. 8; no. 2; pp. 783 - 794 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.02.2019
|
Online Access | Get full text |
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Summary: | Abstract
14‐3‐3ζ has been reported to function as critical regulators of diverse cellular responses. However, the role of 14‐3‐3ζ in gliomas progression remains largely unknown. The expression level of 14‐3‐3ζ and Snail was detected by Western blot analysis and quantitative polymerase chain reaction in different grades of human gliomas. The effect of 14‐3‐3ζ on gliomas progression was measured using cell migration and invasion assay, the colony formation experiment, and
CCK
‐8 assay. The effect of 14‐3‐3ζ on
PI
3K/
AKT
/Snail signaling protein expression levels was tested by Western blotting. Firstly, 14‐3‐3ζ was often up‐regulated in high‐grade gliomas relative to low‐grade gliomas, and this overexpression was significantly related to tumor size, Karnofsky Performance Scale score and weaker disease‐free survival. Secondly, the overexpression of 14‐3‐3ζ promoted gliomas cells proliferation, migration, and invasion. Conversely, the knockdown of 14‐3‐3ζ suppressed gliomas cells proliferation, migration, and invasion. Furthermore, subsequent mechanistic studies showed that 14‐3‐3ζ could activate
PI
3K/
AKT
/
Snail signaling pathway to facilitate gliomas cells proliferation, migration, and invasion. This study shows that the overexpression of 14‐3‐3ζ can promote remarkably gliomas cells proliferation, migration, and invasion by regulating the Snail protein expression through activating
PI
3K/
AKT
signaling, and it may serve as a potential prognostic marker and therapeutic target for gliomas. |
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ISSN: | 2045-7634 2045-7634 |
DOI: | 10.1002/cam4.1950 |