Regulation of Brassinosteroid Signaling by a GSK3/SHAGGY-like Kinase

• Published in: Science (American Association for the Advancement of Science) Vol. 295; no. 5558; pp. 1299 - 1301
• Main Authors: Li, Jianming, Nam, Kyoung Hee
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 15.02.2002; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; BIN2 gene; Biochemistry; Biological and medical sciences; Calcium-Calmodulin-Dependent Protein Kinases - chemistry; Cloning, Molecular; Coding; Complementary DNA; Drosophila Proteins; Economic plant physiology; Enzymes; Flowers & plants; Fundamental and applied biological sciences. Psychology; Genes; Genes, Plant; Genetic mutation; Glycogen Synthase Kinase 3; Growth and development; Growth regulators; GSK3/SHAGGY protein; Humans; Metabolism; Molecular Sequence Data; Mutation; Phenotype; Phenotypes; Phosphorylation; Plant Growth Regulators - metabolism; Plant physiology and development; Plants; Plants (Organisms); Plants, Genetically Modified; Protein kinases; Protein Kinases - chemistry; Protein Kinases - genetics; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases - chemistry; Receptors; Recombinant Fusion Proteins - metabolism; Regulator genes; RNA; Sequence Homology, Amino Acid; Signal Transduction; Steroid hormones; Steroid receptors; Steroids; Steroids - metabolism; Transgenic plants; Visual Aids; United States; Genetic mapping; Enzyme; Growth; Transferases; Structure function relationship; Arabidopsis thaliana; Signal transduction; Brassinosteroid; Gene; Cruciferae; Kinase; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Mutation; Experimental plant
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1065769

GSK3/SHAGGY is a highly conserved serine/threonine kinase implicated in many signaling pathways in eukaryotes. Although many GSK3/SHAGGY-like kinases have been identified in plants, little is known about their functions in plant growth and development. Here we show that the Arabidopsis BRASSI-NOSTEROID-INSENSITIVE 2 (BIN2) gene encodes a GSK3/SHAGGY-like kinase. Gain-of-function mutations within its coding sequence or its overexpression inhibit brassinosteroid (BR) signaling, resulting in plants that resemble BR-deficient and BR-response mutants. In contrast, reduced BIN2 expression via cosuppression partially rescues a weak BR-signaling mutation. Thus, BIN2 acts as a negative regulator to control steroid signaling in plants.