Selective Activation of the B Natriuretic Peptide Receptor by C-Type Natriuretic Peptide (CNP)

• Published in: Science (American Association for the Advancement of Science) Vol. 252; no. 5002; pp. 120 - 123
• Main Authors: Koller, Kerry J., Lowe, David G., Bennett, Gregory L., Minamino, Naoto, Kangawa, Kenji, Matsuo, Hisayuki, Goeddel, David V.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 05.04.1991; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Amino acids; Animals; Atrial Natriuretic Factor - physiology; Biological and medical sciences; Brain; Cell receptors; Cell structures and functions; Cells, Cultured; central nervous system; Cercopithecus aethiops; Cloning, Molecular; COS cells; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Guanylate Cyclase - metabolism; homeostasis; Hormone receptors; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors; Hormones; Humans; Imaging; Kidney cells; Ligands; Medical disorders; Medical research; Molecular and cellular biology; natriuretic hormone (C-type); Natriuretic Peptide, Brain; Natriuretic Peptide, C-Type; Natriuretic peptides; Nerve Tissue Proteins - pharmacology; Neurological disorders; Oocytes; Peptide hormones; Receptors; Receptors, Atrial Natriuretic Factor; Receptors, Cell Surface - physiology; Recombinant Proteins; Signal Transduction; Human; Peptidergic receptor; Signal transduction; Atrial natriuretic peptide; Natriuretic hormone; Hormone receptor complex
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1672777

The natriuretic peptides are hormones that can stimulate natriuretic, diuretic, and vasorelaxant activity in vivo, presumably through the activation of two known cell surface receptor guanylyl cyclases (ANPR-A and ANPR-B). Although atrial natriuretic peptide (ANP) and, to a lesser extent, brain natriuretic peptide (BNP) are efficient activators of the ANPR-A guanylyl cyclase, neither hormone can significantly stimulate ANPR-B. A member of this hormone family, C-type natriuretic peptide (CNP), potently and selectively activated the human ANPR-B guanylyl cyclase. CNP does not increase guanosine 3′,5′-monophosphate accumulation in cells expressing human ANPR-A. The affinity of CNP for ANPR-B is 50- or 500-fold higher than ANP or BNP, respectively. This ligand-receptor pair may be involved in the regulation of fluid homeostasis by the central nervous system.