Sanguinarine modulate gut microbiome and intestinal morphology to enhance growth performance in broilers

• Published in: PloS one Vol. 15; no. 6; p. e0234920
• Main Authors: Liu, Zhu-Ying, Wang, Xiao-Long, Ou, Shu-Qi, Hou, De-Xing, He, Jian-Hua
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 19.06.2020; Public Library of Science (PLoS)
• Subjects: Agriculture; Alkaloids; Animal sciences; Antibiotics; Bacitracin; Bioactive compounds; Biochemistry; Biology and Life Sciences; Birds; Broilers (Chickens); Community structure; Composition; Deoxyribonucleic acid; Diet; Dietary supplements; Digestive system; Digestive tract; DNA; Feces; Feeds; Gastrointestinal tract; Health aspects; Interleukin 4; Intestinal microflora; Intestine; Jejunum; Medicine and Health Sciences; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Morphology; Mucosa; Papaveraceae; Physiological aspects; Poultry; rRNA 16S; Sanguinarine; Sequence analysis; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Urea; Uric acid; Veterinary dietary supplements; Villus; Zoology; China; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0234920

Sanguinarine is a bioactive compound as a quaternary benzophenanthridine alkaloid from plant of the Macleaya cordata, Papaveraceae family. The present study was conducted to investigate the effects of dietary sanguinarine supplementation on growth performance, serum biochemistry parameters, intestinal mucosal morphology and gut microbiome in yellow feathered broilers. Two hundred and seventy 1-d-old female broilers were randomly assigned to 3 treatments (1) Basal diet (NG); (2) Basal diet containing bacitracin methylene disalicylate (50mg/Kg diet) (ANT); ⢠Basal diet containing sanguinarine (0.7 mg/ kg of feed) (SAG). The statistical results showed that dietary sanguinarine supplementation enhanced growth performance and decreased glucose, uric acid as well as urea nitrogen levels of broilers at 28d of age (P<0.05). The 16S rRNA gene sequence analysis revealed that sanguinarine significantly decreased the species from the phyla Bacteroidetes, and increased the species from phyla Firmicutes. Moreover, dietary sanguinarine supplementation improved mucosal morphology to achieve higher ratio of intestinal villus height to crypt depth (P < 0.05), and decreased the concentrations of TNF-[alpha] and IL-4 in jejunum mucosal. This study demonstrated that sanguinarine supplementation in the diet of yellow feathered broilers improved intestinal morphology and microbiota community structure to promote growth performance on 1-28d.