Development of Efficient Packaging Method of Oligodeoxynucleotides by a Condensed Nano Particle in Lipid Envelope Structure

• Published in: Biological & Pharmaceutical Bulletin Vol. 28; no. 10; pp. 1939 - 1942
• Main Authors: Inoue, Kaori, Akita, Hidetaka, Harashima, Hideyoshi, Kogure, Kentaro, Nakamura, Yoshio, Nagatsugi, Fumi, Suhara, Tetsuya, Yamada, Yuma, Sasaki, Shigeki
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.10.2005; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Base Sequence; condensed particle; Drug Carriers; Freezing; Lipids - chemistry; liposome; nano particle; oligodeoxynucleotide; Oligodeoxyribonucleotides - chemistry; packaging; Particle Size
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.28.1939

An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.