Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity

• Published in: Analytical Sciences Vol. 31; no. 7; pp. 715 - 719
• Main Authors: KANNO, Yusuke, INO, Kosuke, INOUE, Kumi Y., SUDA, Atsushi, KUNIKATA, Ryota, MATSUDAIRA, Masahki, SHIKU, Hitoshi, MATSUE, Tomokazu
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2015; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Alkaline phosphatase; Alkaline Phosphatase - metabolism; Analytical Chemistry; Animals; Cell Aggregation; cell analysis; Chemistry; Devices; Electrical measurements; electrochemical imaging; Electrochemistry - instrumentation; Electrodes; Embryoid Bodies - metabolism; Imaging; LSI-based amperometric chip device; Mathematical analysis; Mice; Models, Biological; Oxidation-Reduction; Sensors; Simulation; simulation analysis; Systems Integration; electrochemical imaging; LSI-based amperometric chip device; simulation analysis; cell analysis
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.31.715

In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 μm. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.