Somatostatin Analogs Treated Small Intestinal Neuroendocrine Tumor Patients Circulating MicroRNAs

• Published in: PloS one Vol. 10; no. 5; p. e0125553
• Main Authors: Li, Su-Chen, Khan, Mohid, Caplin, Martyn, Meyer, Tim, Öberg, Kjell, Giandomenico, Valeria
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.05.2015; Public Library of Science (PLoS)
• Subjects: Adult; Aged; Aged, 80 and over; Analogs; Analysis; Biomarkers; Biomarkers, Tumor; Cancer; Care and treatment; Development and progression; Female; Gastroenterology; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genetic aspects; Hospitals; Humans; Intestinal Neoplasms - blood; Intestinal Neoplasms - drug therapy; Intestinal Neoplasms - genetics; Intestinal Neoplasms - pathology; Intestine; Laboratories; Liver; Male; Metabolism; Metastases; Metastasis; MicroRNA; MicroRNAs; MicroRNAs - blood; MicroRNAs - genetics; Middle Aged; miRNA; Neoplasm Grading; Neoplasm Metastasis; Neoplasm Staging; Neuroendocrine tumors; Neuroendocrine Tumors - blood; Neuroendocrine Tumors - drug therapy; Neuroendocrine Tumors - genetics; Neuroendocrine Tumors - pathology; Oncology; Patient outcomes; Patients; Real-Time Polymerase Chain Reaction; Ribonucleic acid; Risk factors; RNA; Science; Somatostatin; Somatostatin - analogs & derivatives; Somatostatin - therapeutic use; Stem cells; Tissues; Tumors; United States
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0125553

We previously detected and investigated nine altered microRNAs in small intestinal neuroendocrine tumor (SI-NET) tissues at different stages of disease. The aims of this study are to: 1) analyze whether SI-NET tissue microRNAs can be also detected in patient serum samples, 2) investigate a potential somatostatin analogs (SSAs) role on microRNA levels regulation in SSA-treated patient samples and 3) elucidate whether the serum microRNA levels in samples collected in different hospitals are predictable and steady. Our results show that tissue microRNAs are detectable in patient serum samples, and miR-96, -182, -183, -196a and -200a levels are lower in SI-NET untreated patients than in SSA-treated patients at all different stages. Conversely, miR-31, -129-5p, -133a and -215 levels do not show any difference in untreated SI-NET patients and SSA-treated patients at all different stages. Our findings also show that miR-200a exhibits an atypical behavior with high levels in both untreated and SSA-treated patients at liver metastasis stage, and unequivocally never at the earlier stages. Serum samples collected in two hospitals keep alike microRNA level pattern, elucidating that the results are not dependent on samples handling. In conclusion, SI-NET tissue microRNAs are always detectable in untreated and SSA-treated patient serum samples, SSAs play an unknown role in eliciting SSA-treated patients' microRNA levels higher than in untreated patients, and this study enlightens that miR-200a might be involved in the liver metastasis during SI-NET progression.