Complement-activating IgM enhances the humoral but not the T cell immune response in mice

• Published in: PloS one Vol. 8; no. 11; p. e81299
• Main Authors: Ding, Zhoujie, Bergman, Anna, Rutemark, Christian, Ouchida, Rika, Ohno, Hiroshi, Wang, Ji-Yang, Heyman, Birgitta
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.11.2013; Public Library of Science (PLoS)
• Subjects: Animals; Antibodies; Antigens; B cells; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Biochemistry; Blood; Blood cells; Carrier Proteins - immunology; Carrier Proteins - metabolism; CD4 antigen; CD44 antigen; Cell activation; Cell division; Complement; Complement activation; Complement Activation - immunology; Complement component C3; Complement System Proteins - immunology; Dendritic cells; Divergence; Erythrocytes; Experiments; Fc receptors; Gene Knock-In Techniques; Genetic transformation; Immune response; Immune response (cell-mediated); Immune response (humoral); Immune system; Immunity, Humoral; Immunization; Immunoglobulin M; Immunoglobulin M - immunology; Immunoglobulin M - metabolism; Immunoglobulins; Immunologic factors; Immunology; Laboratories; LFA-1 antigen; Lymphocytes; Lymphocytes B; Lymphocytes T; Membrane Proteins - immunology; Membrane Proteins - metabolism; Mice; Mutation; Receptors, Fc - immunology; Receptors, Fc - metabolism; Sheep; Sheep red blood cells; T cell receptors; T cells; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Transformation; Sweden; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0081299

IgM antibodies specific for a certain antigen can enhance antibody responses when administered together with this antigen, a process believed to require complement activation by IgM. However, recent data show that a knock-in mouse strain, Cμ13, which only produces IgM unable to activate complement, has normal antibody responses. Moreover, the recently discovered murine IgM Fc receptor (FcµR or TOSO/FAIM3) was shown to affect antibody responses. This prompted the re-investigation of whether complement activation by specific IgM is indeed required for enhancement of antibody responses and whether the mutation in Cµ13 IgM also caused impaired binding to FcµR. The results show that IgM from Cµ13 and wildtype mice bound equally well to the murine FcµR. In spite of this, specific Cμ13 IgM administered together with sheep red blood cells or keyhole limpet hemocyanine was a very poor enhancer of the antibody and germinal center responses as compared with wildtype IgM. Within seconds after immunization, wildtype IgM induced deposition of C3 on sheep red blood cells in the blood. IgM which efficiently enhanced the T-dependent humoral immune response had no effect on activation of specific CD4(+) T cells as measured by cell numbers, cell division, blast transformation, or expression of the activation markers LFA-1 and CD44 in vivo. These observations confirm the importance of complement for the ability of specific IgM to enhance antibody responses and suggest that there is a divergence between the regulation of T- and B-cell responses by IgM.