Attachment and Infection to MA104 Cells of Avian Rotaviruses Require the Presence of Sialic Acid on the Cell Surface

• Published in: Journal of Veterinary Medical Science Vol. 66; no. 4; pp. 461 - 463
• Main Authors: MINAMOTO, Nobuyuki, SUGIYAMA, Makoto, MORI, Yoshio, GOTO, Kazuo, UEMUKAI, Hiroko, ITO, Naoto
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2004; Japan Science and Technology Agency
• Subjects: Animals; Birds - virology; Blotting, Western; Capsid Proteins - genetics; Capsid Proteins - metabolism; Cells, Cultured; DNA Primers; DNA, Complementary - genetics; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Glutathione Transferase - metabolism; Neuraminidase - pharmacology; Protein Binding - drug effects; Receptors, Cell Surface - metabolism; Rotavirus; Rotavirus - metabolism; sialic acid; VP8
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.66.461

To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 cells with neuraminidase greatly reduced the infection by all of the four avian rotavirus strains. Binding of the cell-attachment protein, purified VP8 expressed in bacteria, of strain PO-13 to MA104 cells was also inhibited by pretreatment of cells with neuraminidase. These findings suggest that avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA 104 cells.