Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen conven...

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Published inThe Canadian journal of infectious diseases & medical microbiology Vol. 2016; no. 2016; pp. 1 - 9
Main Authors Wang, Chengming, Wu, Hongzhuan, Yang, Zhangping, Mao, Yongjiang, Yang, Yi, Luo, Qinghua, Zhang, Jilei, Kelly, Patrick, Qiu, Haixiang, Li, Jing
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2016
John Wiley & Sons, Inc
Hindawi Limited
Subjects
Anaplasma
Anaplasmosis
Buffalo
Cattle
Deoxyribonucleic acid
Diagnosis
DNA
Domestic animals
Economic importance
Ehrlichia
Ehrlichiosis
Fluorescence resonance energy transfer
Genetic aspects
Goats
Health aspects
Infectious diseases
Polymerase chain reaction
Provinces
Ribosomal RNA
rRNA 16S
Ruminants
Sheep
University colleges
Veterinary colleges
Veterinary medicine
China
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Summary:Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma spp. and Ehrlichia spp. Positive samples were further analyzed with a standard PCR for the gltA. Anaplasma spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). Ehrlichia spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of gltA PCR products showed that A. marginale, A. ovis, Ehrlichia canis, and Ehrlichia sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. BL126-13 (KJ410243), and Anaplasma sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of Anaplasma spp. and Ehrlichia spp.
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Academic Editor: Matilde J. Coello
ISSN:1712-9532
1918-1493
DOI:10.1155/2016/9183861