Genetically engineered resistance to dengue-2 virus transmission in mosquitoes

• Published in: Science (American Association for the Advancement of Science) Vol. 272; no. 5263; pp. 884 - 886
• Main Authors: Olson, K.E. (Colorado State University, Fort Collins, CO.), Higgs, S, Gaines, P.J, Powers, A.M, Davis, B.S, Kamrud, K.I, Carlson, J.O, Blair, C.D, Beaty, B.J
• Format: Journal Article
• Language: English
• Published: United States American Society for the Advancement of Science 10.05.1996; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Aedes - genetics; Aedes - virology; AEDES AEGYPTI; Animals; Antisense RNA; ARN; Coding; Complementary DNA; Control; Culicidae; Dengue; Dengue - transmission; Dengue fever; Dengue virus; Dengue Virus - genetics; Dengue Virus - physiology; dengue virus 2; Dengue virus type 2; Dengue viruses; Digestive System - virology; Diptera; Disease transmission; Female; Genetic aspects; Genetic Engineering; Genetic Vectors; Genetics; GENIE GENETIQUE; Genome, Viral; GLANDE SALIVAIRE; GLANDULAS SALIVALES; Humans; Infections; INGENIERIA GENETICA; INHIBICION; INHIBITION; Insect carriers of disease; Insect Vectors - genetics; Insect Vectors - virology; Insects; Insects as carriers of disease; Messenger RNA; Mosquitoes; Mosquitoes as carriers of disease; Mosquitos; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; RNA; RNA, Antisense - genetics; Saliva; Salivary glands; Salivary Glands - virology; Sindbis virus; Sindbis Virus - genetics; TRANSMISION DE ENFERMEDADES; TRANSMISSION DES MALADIES; VECTEUR DE MALADIE; VECTORES; Virus Replication; Viruses
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.272.5263.884

The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus