Fenoxycarb exposure disrupted the reproductive success of the amphipod Gammarus fossarum with limited effects on the lipid profile

• Published in: PloS one Vol. 13; no. 4; p. e0196461
• Main Authors: Arambourou, Hélène, Fuertes, Inmaculada, Vulliet, Emmanuelle, Daniele, Gaëlle, Noury, Patrice, Delorme, Nicolas, Abbaci, Khedidja, Barata, Carlos
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.04.2018; Public Library of Science (PLoS)
• Subjects: Amphipoda; Analysis; Analytical chemistry; Animal reproduction; Arthropods; Behavior; Bernard, Claude (1813-1878); Biology and Life Sciences; Breeding success; Chemical Sciences; Crustacea; Crustaceans; Daphnia magna; Eggs; Embryos; Energy reserves; Exposure; Females; Fenoxycarb; Fertilization; Freshwater crustaceans; Gammaridae; Gammarus fossarum; Genetic aspects; Glycogen; Hormones; Immune response; Immune system; Insect pests; Insecticides; Insects; Laboratories; Lipid metabolism; Lipids; Males; Oogenesis; Pests; Phenoloxidase; Physiological aspects; Proteins; Reproduction; Success; France; Spain; fenoxycarb; glycogen; lipid; monophenol monooxygenase; protein
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0196461

Insect growth regulator insecticides mimic the action of hormones on the growth and development of insect pests. However, they can affect the development of non-target arthropods. In the present study, we tested the effects of the growth regulator insecticide fenoxycarb on several endpoints in the freshwater crustacean Gammarus fossarum (Amphipoda). Females carrying embryos in their open brood pouch were exposed to 50 μg L-1 fenoxycarb throughout the entire oogenesis (i.e. 21 days). After exposure, newborn individuals from exposed embryos were removed from the maternal open brood pouch for lipidomic analysis, while males were added to assess the reproductive success. After fertilization, the lipid profile, energy reserve content (lipids, proteins and glycogen), and activity of phenoloxidase - an enzyme involved in the immune response - were measured in females. No significant effect of fenoxycarb exposure was observed on the lipid profile of both newborn individuals and females, while reproductive success was severely impaired in exposed females. Particularly, precopulatory behavior was significantly reduced and fertilized eggs were unviable. This study highlighted the deleterious effects of the insect growth regulator fenoxycarb on gammarid reproduction, which could have severe repercussions on population dynamics.