A protocol to assess insect resistance to heat waves, applied to bumblebees (Bombus Latreille, 1802)

• Published in: PloS one Vol. 10; no. 3; p. e0118591
• Main Authors: Martinet, Baptiste, Lecocq, Thomas, Smet, Jérémy, Rasmont, Pierre
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.03.2015; Public Library of Science (PLoS)
• Subjects: Accuracy; Alpine environments; Animals; Bees - physiology; Bioclimatology; Biodiversity; Biological Assay - instrumentation; Biological Assay - methods; Bumblebees; Climate Change; Datasets; Ecology, environment; Endangered & extinct species; Genetic aspects; Global temperature changes; Heat resistance; Heat stress; Heat tolerance; Heat waves; Heat-Shock Response; Hot Temperature; Humidity; Insecta; Insects; Laboratories; Life Sciences; Male; Physiological aspects; Polar environments; Power supply; Species; Stress concentration; Temperature extremes; Thermal resistance; Wave resistance; Zoology; Belgium; United States
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0118591

Insect decline results from numerous interacting factors including climate change. One of the major phenomena related to climate change is the increase of the frequency of extreme events such as heat waves. Since heat waves are suspected to dramatically increase insect mortality, there is an urgent need to assess their potential impact. Here, we determined and compared the resistance to heat waves of insects under hyperthermic stress through their time before heat stupor (THS) when they are exposed to an extreme temperature (40°C). For this, we used a new experimental standardised device available in the field or in locations close to the field collecting sites. We applied this approach on different Arctic, Boreo-Alpine and Widespread bumblebee species in order to predict consequences of heat waves. Our results show a heat resistance gradient: the heat stress resistance of species with a centred arctic distribution is weaker than the heat resistance of the Boreo-Alpine species with a larger distribution which is itself lower than the heat stress resistance of the ubiquitous species.