Lactoferrin Adsorbed onto Biomimetic Hydroxyapatite Nanocrystals Controlling - In Vivo - the Helicobacter pylori Infection

• Published in: PloS one Vol. 11; no. 7; p. e0158646
• Main Authors: Fulgione, Andrea, Nocerino, Nunzia, Iannaccone, Marco, Roperto, Sante, Capuano, Federico, Roveri, Norberto, Lelli, Marco, Crasto, Antonio, Calogero, Armando, Pilloni, Argenia Paola, Capparelli, Rosanna
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.07.2016; Public Library of Science (PLoS)
• Subjects: Adsorption; Agriculture; Animals; Anti-Infective Agents - administration & dosage; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antibiotics; Antioxidants; Antioxidants (Nutrients); Aqueous solutions; Biology and Life Sciences; Biomimetic Materials - chemistry; Biomimetics; Care and treatment; Complications and side effects; Crystals; Culture Media, Conditioned - pharmacology; Cytokines - metabolism; Drug Delivery Systems - methods; Durapatite - chemistry; Engineering and Technology; Health aspects; Helicobacter infections; Helicobacter Infections - microbiology; Helicobacter Infections - prevention & control; Helicobacter pylori; Helicobacter pylori - drug effects; Helicobacter pylori - physiology; Host-Pathogen Interactions - drug effects; Hydroxyapatite; Immunomodulation; In vivo methods and tests; Infections; Lactobacillus paracasei; Lactobacillus paracasei - physiology; Lactoferrin; Lactoferrin - administration & dosage; Lactoferrin - chemistry; Lactoferrin - pharmacology; Medicine and Health Sciences; Mice, Inbred BALB C; Microscopy; Microscopy, Electron, Transmission; Nanocrystals; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Physiological aspects; Probiotics; Probiotics - administration & dosage; Probiotics - pharmacology; Proteins; Salmonella; Salmonella Typhimurium; Side effects; Stomach - drug effects; Stomach - metabolism; Stomach - microbiology; Therapy; Treatment Outcome; X-Ray Diffraction; Italy; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0158646

The resistance of Helicobacter pylori to the antibiotic therapy poses the problem to discover new therapeutic approaches. Recently it has been stated that antibacterial, immunomodulatory, and antioxidant properties of lactoferrin are increased when this protein is surface-linked to biomimetic hydroxyapatite nanocrystals. Based on these knowledge, the aim of the study was to investigate the efficacy of lactoferrin delivered by biomimetic hydroxyapatite nanoparticles with cell free supernatant from probiotic Lactobacillus paracasei as an alternative therapy against Helicobacter pylori infection. Antibacterial and antinflammatory properties, humoral antibody induction, histopathological analysis and absence of side effects were evaluated in both in vitro and in vivo studies. The tests carried out have been demonstrated better performance of lactoferrin delivered by biomimetic hydroxyapatite nanoparticles combined with cell free supernatant from probiotic Lactobacillus paracasei compared to both lactoferrin and probiotic alone or pooled. These findings indicate the effectiveness and safety of our proposed therapy as alternative treatment for Helicobacter pylori infection.