Analysis of the In Vivo Turnover of CD4+ T-Cell Subsets in Chronically SIV-Infected Sooty Mangabeys

• Published in: PloS one Vol. 11; no. 5; p. e0156352
• Main Authors: Ortiz, Alexandra M, Carnathan, Diane G, Yu, Joana, Sheehan, Katherine M, Kim, Peter, Reynaldi, Arnold, Vanderford, Thomas H, Klatt, Nichole R, Brenchley, Jason M, Davenport, Miles P, Silvestri, Guido
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.05.2016; Public Library of Science (PLoS)
• Subjects: Acquired immune deficiency syndrome; AIDS; Analysis; Animals; Antigens; Biology; Biology and Life Sciences; Care and treatment; CD4 antigen; CD4-Positive T-Lymphocytes - cytology; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - virology; Cell growth; Cell proliferation; Cercocebus atys; Cytometry; Development and progression; Disease Progression; Flow cytometry; HIV; Homeostasis; Human immunodeficiency virus; Immune response; Immunological memory; Immunology; Immunoregulation; Infections; Laboratory animals; Lymphocyte Activation; Lymphocytes; Lymphocytes T; Medicine and Health Sciences; Memory cells; Prevention; Primates; Research and Analysis Methods; Simian Acquired Immunodeficiency Syndrome - pathology; Simian Acquired Immunodeficiency Syndrome - virology; Simian immunodeficiency virus; Simian Immunodeficiency Virus - immunology; Sooty mangabey; Swine influenza virus; T cells; T-Lymphocyte Subsets - cytology; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - virology; Therapeutic applications; Viral Load; Viruses; Atlanta Georgia; Georgia; Australia; United States > US; Maryland
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0156352

Aberrant turnover of memory CD4+ T-cells is central to Acquired Immunodeficiency Syndrome (AIDS) progression. Understanding the relationship between the turnover of CD4+ subsets and immunological homeostasis during simian immunodeficiency virus (SIV) infection in natural hosts may provide insight into mechanisms of immune regulation that may serve as models for therapeutic intervention in Human Immunodeficiency Virus (HIV)-infected persons. Sooty mangabeys (SMs) have naturally evolved with SIV to avoid AIDS progression while maintaining healthy peripheral CD4+ T-cell counts and thus represent a model by which therapeutic interventions for AIDS progression might be elucidated. To assess the relationship between the turnover of CD4+ subsets and immunological homeostasis during SIV infection in non-progressive hosts, we treated 6 SIV-uninfected and 9 SIV-infected SMs with 2'-bromo-5'-deoxyuridine (BrdU) for 14 days and longitudinally assessed CD4+ T-cell subset turnover by polychromatic flow cytometry. We observed that, in SIV-infected SMs, turnover of CD4+ T-cell naïve and central, transitional, and effector memory subsets is comparable to that in uninfected animals. Comparable turnover of CD4+ T-cell subsets irrespective of SIV-infection status likely contributes to the lack of aberrant immune activation and disease progression observed after infection in non-progressive hosts.