Polymorphisms and features of cytomegalovirus UL144 and UL146 in congenitally infected neonates with hepatic involvement

• Published in: PloS one Vol. 12; no. 2; p. e0171959
• Main Authors: Guo, Gangqiang, Zhang, Liang, Ye, Sisi, Hu, Yingying, Li, Baoqing, Sun, Xiangwei, Mao, Chenchen, Xu, Jianfeng, Chen, Yiping, Zhang, Lifang, Xue, Xiangyang
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.02.2017; Public Library of Science (PLoS)
• Subjects: Alanine; Alanine transaminase; Alanine Transaminase - blood; Aspartate; Aspartate aminotransferase; Aspartate Aminotransferases - blood; Biology and life sciences; Care and treatment; Chemokines; Chemokines, CXC - genetics; Children & youth; Congenital diseases; Cytomegalovirus; Cytomegalovirus - genetics; Cytomegalovirus infections; Cytomegalovirus Infections - congenital; Disease; Gene sequencing; Genes; Genetic aspects; Genetic polymorphisms; Genotypes; Glycoproteins; Health aspects; Hearing loss; Hepatitis; Hepatitis, Viral, Human - etiology; Hepatitis, Viral, Human - virology; Herpesviridae; Hospitals; Humans; Immunoglobulin G; Immunoglobulin M; Immunology; Infant, Newborn; Infections; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - blood; Laboratories; Lactate dehydrogenase; Lactic acid; Liver; Medicine; Medicine and Health Sciences; Membrane Glycoproteins - genetics; Neonates; Neutrophils; Newborn babies; Newborn infants; Phylogeny; Polymerase Chain Reaction; Polymorphism, Genetic - genetics; Population; Research and Analysis Methods; Sequence Analysis, DNA; Surgery; Urine; Viral Proteins - genetics; Virology; Viruses; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0171959

Human cytomegalovirus is a significant agent of hepatic involvement in neonates. In this study, we investigated the polymorphisms and features of the viral genes UL144 and UL146 as well as their significance to congenital hepatic involvement. In 79 neonates with congenital cytomegalovirus infection and hepatic involvement, full length UL144 and UL146 were successfully amplified in 73.42% and 60.76% of cases, respectively. Sequencing indicated that both genes were hypervariable. Notably, UL144 genotype B was highly associated with aspartate aminotransferase (P = 0.028) and lactate dehydrogenase (P = 0.046). Similarly, UL146 genotype G1 and G13 were significantly associated with CMV IgM (P = 0.026), CMV IgG (P = 0.034), alanine aminotransferase (P = 0.019), and aspartate aminotransferase (P = 0.032). In conclusion, dominant UL144 (genotype B) and UL146 (genotype G1 and G13) genotypes are associated with elevated levels of enzymes and CMV IgM and IgG of cytomegalovirus infection.