Bioactive Polymeric Nanoparticles for Periodontal Therapy
to design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease. PolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body flu...
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Published in | PloS one Vol. 11; no. 11; p. e0166217 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Public Library of Science
07.11.2016
Public Library of Science (PLoS) |
Subjects | |
Online Access | Get full text |
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Summary: | to design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease.
PolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.
Precipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect.
The ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conceptualization: RO MT MA CAAR AMC. Data curation: CAAR. Formal analysis: MA MT RO. Funding acquisition: MT RO MA. Investigation: RO MT MA CAAR AMC. Methodology: RO MT MA CAAR AMC. Project administration: RO MT MA. Resources: AMC CAAR. Software: RO MA AMC. Supervision: RO MA MT. Validation: RO MT MA CAAR AMC. Visualization: RO MT. Writing – original draft: RO MT MA. Writing – review & editing: RO MT MA CAAR AMC. Competing Interests: The authors have declared that no competing interests exist. |
ISSN: | 1932-6203 1932-6203 |
DOI: | 10.1371/journal.pone.0166217 |