Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm

• Published in: PloS one Vol. 12; no. 2; p. e0172691
• Main Authors: Smorodinova, Natalia, Bláha, Martin, Melenovský, Vojtěch, Rozsívalová, Karolína, Přidal, Jaromír, Ďurišová, Mária, Pirk, Jan, Kautzner, Josef, Kučera, Tomáš
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.02.2017; Public Library of Science (PLoS)
• Subjects: Aged; Arrhythmia; Arrhythmia, Sinus - immunology; Arrhythmia, Sinus - pathology; Arrhythmia, Sinus - physiopathology; Atrial fibrillation; Atrial Fibrillation - immunology; Atrial Fibrillation - pathology; Atrial Fibrillation - physiopathology; B-Lymphocytes - immunology; B-Lymphocytes - pathology; Beta cells; Biology and Life Sciences; Body size; Cardiac arrhythmia; Cardiology; Cardiomyocytes; Cardiomyopathy; Cardiovascular disease; CD20 antigen; CD3 antigen; CD45 antigen; Cell Count; Cell populations; Cell Shape; Coronary vessels; Cytokines; Cytology; DC-SIGN protein; Dendritic cells; Diabetes mellitus; Embryology; Female; Fibrillation; Genetic aspects; Heart Atria - immunology; Heart Atria - pathology; Heart Atria - physiopathology; Heart failure; Heart surgery; Histology; Humans; Hypertension; Immunohistochemistry; Infiltration; Inflammation; Lymphocytes; Lymphocytes T; Macrophages; Male; Markers; Mast cells; Mast Cells - immunology; Mast Cells - pathology; Medicine; Medicine and Health Sciences; Middle Aged; Monocytes; Morphology; Myocardium; Myocardium - immunology; Myocardium - pathology; Pathogenesis; Patients; Populations; Regurgitation; Rhythm; Sinus; Stroke; Studies; Surgery; T cells; T-Lymphocytes - immunology; T-Lymphocytes - pathology; Czech Republic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0172691

Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. The goal of this study was to characterize morphologically and functionally CD45-positive inflammatory cell populations in atrial myocardium of patients with AF as compared to sinus rhythm (SR). We examined 46 subjects (19 with AF, and 27 in SR) undergoing coronary bypass or valve surgery. Peroperative bioptic samples of the left and the right atrial tissue were examined using immunohistochemistry. The number of CD3+ T-lymphocytes and CD68-KP1+ cells were elevated in the left atrial myocardium of patients with AF compared to those in SR. Immune cell infiltration of LA was related to the rhythm, but not to age, body size, LA size, mitral regurgitation grade, type of surgery, systemic markers of inflammation or presence of diabetes or hypertension. Most of CD68-KP1+ cells corresponded to dendritic cell population based on their morphology and immunoreactivity for DC-SIGN. The numbers of mast cells and CD20+ B-lymphocytes did not differ between AF and SR patients. No foci of inflammation were detected in any sample. An immunohistochemical analysis of samples from patients undergoing open heart surgery showed moderate and site-specific increase of inflammatory cells in the atrial myocardium of patients with AF compared to those in SR, with prevailing population of monocyte-macrophage lineage. These cells and their cytokine products may play a role in atrial remodeling and AF persistence.