Comprehensive Virus Detection Using Next Generation Sequencing in Grapevine Vascular Tissues of Plants Obtained from the Wine Regions of Bohemia and Moravia (Czech Republic)

• Published in: PloS one Vol. 11; no. 12; p. e0167966
• Main Authors: Eichmeier, Aleš, Komínková, Marcela, Komínek, Petr, Baránek, Miroslav
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.12.2016; Public Library of Science (PLoS)
• Subjects: Analysis; Biology and Life Sciences; Czech Republic; DNA sequencing; DNA, Viral - chemistry; Farms; Gene sequencing; Genetics; Grapevines; High-Throughput Nucleotide Sequencing - methods; Horticulture; Infections; Microorganisms; Nucleotide sequencing; Pathogens; Plant tissues; Plant viruses; Plant Viruses - isolation & purification; Research and Analysis Methods; Ribonucleic acid; RNA; Sequence Analysis, DNA - methods; Vascular system of plants; Vineyards; Viroids; Viroids - isolation & purification; Viruses; Vitaceae; Vitis - virology; Vitis vinifera; Wine; Wineries & vineyards; Wines; Czech Republic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0167966

Comprehensive next generation sequencing virus detection was used to detect the whole spectrum of viruses and viroids in selected grapevines from the Czech Republic. The novel NGS approach was based on sequencing libraries of small RNA isolated from grapevine vascular tissues. Eight previously partially-characterized grapevines of diverse varieties were selected and subjected to analysis: Chardonnay, Laurot, Guzal Kara, and rootstock Kober 125AA from the Moravia wine-producing region; plus Müller-Thurgau and Pinot Noir from the Bohemia wine-producing region, both in the Czech Republic. Using next generation sequencing of small RNA, the presence of 8 viruses and 2 viroids were detected in a set of eight grapevines; therefore, confirming the high effectiveness of the technique in plant virology and producing results supporting previous data on multiple infected grapevines in Czech vineyards. Among the pathogens detected, the Grapevine rupestris vein feathering virus and Grapevine yellow speckle viroid 1 were recorded in the Czech Republic for the first time.