Exploring the Shift in Structure and Function of Microbial Communities Performing Biological Phosphorus Removal

• Published in: PloS one Vol. 11; no. 8; p. e0161506
• Main Authors: Mao, Yanping, Wang, Zhiping, Li, Liguan, Jiang, Xiaotao, Zhang, Xuxiang, Ren, Hongqiang, Zhang, Tong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.08.2016; Public Library of Science (PLoS)
• Subjects: Acetic acid; Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - metabolism; Bacteria; Batch Cell Culture Techniques; Batch reactors; Betaproteobacteria - classification; Betaproteobacteria - genetics; Betaproteobacteria - metabolism; Bioaccumulation; Biodegradation, Environmental; Bioinformatics; Biology and Life Sciences; Bioreactors; Biotechnology; Candidatus Accumulibacter phosphatis; Candidatus Competibacter phosphatis; Carbon; Carbon Cycle - genetics; Carbon fixation; Civil engineering; Communities; Environmental aspects; Genes; Genome, Bacterial; Genomes; Genomic analysis; Glycogen; Humans; Hydrogen-Ion Concentration; Laboratories; Metabolism; Microbial activity; Microbial Consortia - genetics; Microbiomes; Microorganisms; Nitrogen - metabolism; Organisms; pH effects; Phosphates; Phosphorus; Phosphorus (Nutrient); Phosphorus - isolation & purification; Phosphorus - metabolism; Phosphorus removal; Phylogeny; Physical Sciences; Physiological aspects; Polyphosphates; Populations; Proteobacteria; Research and Analysis Methods; RNA sequencing; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequencing batch reactor; Structure-function relationships; Waste Water - chemistry; Water Pollutants, Chemical - isolation & purification; Water Pollutants, Chemical - metabolism; Water Purification - methods
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0161506

A sequencing batch reactor fed mainly by acetate was operated to perform enhanced biological phosphorus removal (EBPR). A short-term pH shock from 7.0 to 6.0 led to a complete loss of phosphate-removing capability and a drastic change of microbial communities. 16S rRNA gene pyrosequencing showed that large proportions of glycogen accumulating organisms (GAOs) (accounted for 16% of bacteria) bloomed, including Candidatus Competibacter phosphatis and Defluviicoccus-related tetrad-forming organism, causing deteriorated EBPR performance. The EBPR performance recovered with time and the dominant Candidatus Accumulibacter (Accumulibacter) clades shifted from Clade IIC to IIA while GAOs populations shrank significantly. The Accumulibacter population variation provided a good opportunity for genome binning using a bi-dimensional coverage method, and a genome of Accumulibacter Clade IIC was well retrieved with over 90% completeness. Comparative genomic analysis demonstrated that Accumulibacter clades had different abilities in nitrogen metabolism and carbon fixation, which shed light on enriching different Accumulibacter populations selectively.