Time-Course Small RNA Profiling Reveals Rice miRNAs and Their Target Genes in Response to Rice Stripe Virus Infection

• Published in: PloS one Vol. 11; no. 9; p. e0162319
• Main Authors: Lian, Sen, Cho, Won Kyong, Kim, Sang-Min, Choi, Hoseong, Kim, Kook-Hyung
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.09.2016; Public Library of Science (PLoS)
• Subjects: Agricultural biotechnology; Agriculture; Analysis; Arabidopsis; Biology and life sciences; Crop diseases; Defense mechanisms; Epidemics; Gene expression; Gene sequencing; Genes; Genes, Plant; Genomes; Genomics; Health aspects; Infection; Infections; Life sciences; MicroRNA; MicroRNAs; MicroRNAs - genetics; miRNA; NBS-LRR gene; Next-generation sequencing; Oryza; Oryza - genetics; Oryza sativa; Plant Diseases - genetics; Plant Viruses - genetics; Research and Analysis Methods; Ribonucleic acid; Rice; Rice stripe virus; RNA; RNA sequencing; RNA, Viral - genetics; Studies; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0162319

It has been known that many microRNAs (miRNAs) are involved in the regulation for the plant development and defense mechanism by regulating the expression of the target gene. Several previous studies has demonstrated functional roles of miRNAs in antiviral defense mechanisms. In this study, we employed high-throughput sequencing technology to identify rice miRNAs upon rice stripe virus (RSV) infection at three different time points. Six libraries from mock and RSV-infected samples were subjected for small RNA sequencing. Bioinformatic analyses revealed 374 known miRNAs and 19 novel miRNAs. Expression of most identified miRNAs was not dramatically changed at 3 days post infection (dpi) and 7 dpi by RSV infection. However, many numbers of miRNAs were up-regulated in mock and RSV-infected samples at 15 dpi by RSV infection. Moreover, expression profiles of identified miRNAs revealed that only few numbers of miRNAs were strongly regulated by RSV infection. In addition, 15 resistance genes were targets of six miRNAs suggesting that those identified miRNAs and 15 NBS-LRR resistance genes might be involved in RSV infection. Taken together, our results provide novel insight into the dynamic expression profiles of rice miRNAs upon RSV infection and clues for the understanding of the regulatory roles of miRNAs via time-course.