Iron-restricted pair-feeding affects renal damage in rats with chronic kidney disease

• Published in: PloS one Vol. 12; no. 2; p. e0172157
• Main Authors: Naito, Yoshiro, Senchi, Aya, Sawada, Hisashi, Oboshi, Makiko, Horimatsu, Tetsuo, Okuno, Keisuke, Yasumura, Seiki, Ishihara, Masaharu, Masuyama, Tohru
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.02.2017; Public Library of Science (PLoS)
• Subjects: Anemia; Animal experimentation; Animals; Appetite; Appetite loss; Biology and Life Sciences; Caloric Restriction; Cardiovascular disease; Cellulose; Chronic kidney failure; Coronary vessels; Damage prevention; Diet; Dietary restrictions; Disease Models, Animal; Eating; End-stage renal disease; Feeding; Food; Food intake; Gene expression; Gene Expression Regulation; Hypertension; Internal medicine; Iron; Iron - deficiency; Kidney - injuries; Kidney - metabolism; Kidney - physiopathology; Kidney diseases; Kinases; Laboratory animals; Male; Medicine; Medicine and Health Sciences; Nephrectomy; Nutrient deficiency; Prevalence studies (Epidemiology); Proteinuria; Rats; Rats, Sprague-Dawley; Receptors, Mineralocorticoid - biosynthesis; Renal Insufficiency, Chronic - metabolism; Renal Insufficiency, Chronic - pathology; Renal Insufficiency, Chronic - physiopathology; Risk factors; Rodents; Surgery; Systematic review; Tumor necrosis factor-TNF; Urine; Japan; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0172157

We have previously shown that dietary iron restriction prevents the development of renal damage in a rat model of chronic kidney disease (CKD). However, iron deficiency is associated with appetite loss. In addition, calorie restriction is reported to prevent the development of end-stage renal pathology in CKD rats. Thus, the beneficial effect of iron restriction on renal damage may depend on calorie restriction. Here, we investigate the effect of pair-feeding iron restriction on renal damage in a rat model of CKD. First, to determine the amount of food intake, Sprague-Dawley (SD) rats were randomly given an ad libitum normal diet or an iron-restricted diet, and the food intake was measured. Second, CKD was induced by a 5/6 nephrectomy in SD rats, and CKD rats were given either a pair-feeding normal or iron-restricted diet. Food intake was reduced in the iron-restricted diet group compared to the normal diet group of SD rats for 16 weeks (mean food intake; normal diet group and iron-restricted diet group: 25 and 20 g/day, respectively). Based on the initial experiments, CKD rats received either a pair-feeding normal or iron-restricted diet (20 g/day) for 16 weeks. Importantly, pair-feeding iron restriction prevented the development of proteinuria, glomerulosclerosis, and tubulointerstitial damage in CKD rats. Interestingly, pair-feeding iron restriction attenuated renal expression of nuclear mineralocorticoid receptor in CKD rats. Pair-feeding iron restriction affected renal damage in a rat model of CKD.