Meta-QTL for resistance to white mold in common bean

• Published in: PloS one Vol. 12; no. 2; p. e0171685
• Main Authors: Vasconcellos, Renato C C, Oraguzie, O Blessing, Soler, Alvaro, Arkwazee, Haidar, Myers, James R, Ferreira, Juan J, Song, Qijian, McClean, Phil, Miklas, Phillip N
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.02.2017; Public Library of Science (PLoS)
• Subjects: Agricultural production; Arabidopsis; Ascomycota - genetics; Biology and Life Sciences; Cell walls; Chromosome Mapping; Chromosomes, Plant - chemistry; Chromosomes, Plant - genetics; Common beans; Confidence intervals; Crop diseases; Cultivars; Disease control; Disease resistance (Plants); Disease Resistance - genetics; Fungi; Genetic Linkage; Genomes; Genomics; Genotype; Germplasm; Glycine max; Heritability; Horticulture; Inbreeding; Legumes; Meta-analysis; Mold; Molds (Fungi); Peroxidase; Peroxidase - genetics; Peroxidase - metabolism; Phaseolus - genetics; Phaseolus - microbiology; Physiological aspects; Physiology; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Polymorphism, Single Nucleotide; Populations; Quantitative Trait Loci; Research and Analysis Methods; Sclerotinia sclerotiorum; Soybeans; Transcription Factors - genetics; Transcription Factors - metabolism; White mold; Oregon; United States > US; North Dakota
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0171685

White mold, caused by the fungus Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease that limits common bean production and quality worldwide. The host-pathogen interaction is complex, with partial resistance in the host inherited as a quantitative trait with low to moderate heritability. Our objective was to identify meta-QTL conditioning partial resistance to white mold from individual QTL identified across multiple populations and environments. The physical positions for 37 individual QTL were identified across 14 recombinant inbred bi-parental populations (six new, three re-genotyped, and five from the literature). A meta-QTL analysis of the 37 QTL was conducted using the genetic linkage map of Stampede x Red Hawk population as the reference. The 37 QTL condensed into 17 named loci (12 previously named and five new) of which nine were defined as meta-QTL WM1.1, WM2.2, WM3.1, WM5.4, WM6.2, WM7.1, WM7.4, WM7.5, and WM8.3. The nine meta-QTL had confidence intervals ranging from 0.65 to 9.41 Mb. Candidate genes shown to express under S. sclerotiorum infection in other studies, including cell wall receptor kinase, COI1, ethylene responsive transcription factor, peroxidase, and MYB transcription factor, were found within the confidence interval for five of the meta-QTL. The nine meta-QTL are recommended as potential targets for MAS for partial resistance to white mold in common bean.