Exploration of inhibitory mechanisms of curcumin in lung cancer metastasis using a miRNA- transcription factor-target gene network

• Published in: PloS one Vol. 12; no. 2; p. e0172470
• Main Authors: Jiao, De-min, Yan, Li, Wang, Li-shan, Hu, Hui-zhen, Tang, Xia-li, Chen, Jun, Wang, Jian, Li, You, Chen, Qing-yong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.02.2017; Public Library of Science (PLoS)
• Subjects: Antineoplastic Agents - pharmacology; Apoptosis; Biochemistry; Biology; Biology and life sciences; Bone cancer; Cancer; Cancer metastasis; Carcinoma, Non-Small-Cell Lung - drug therapy; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Care and treatment; Cell cycle; Cell growth; Cell Movement - drug effects; Computer and Information Sciences; Control; Curcumin; Curcumin - pharmacology; Data bases; Disease prevention; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene Regulatory Networks - drug effects; Genes; Genetic aspects; Health aspects; Humans; Kinases; Literature reviews; Lung - drug effects; Lung - pathology; Lung cancer; Lung diseases; Lung Neoplasms - drug therapy; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Medical research; Medicine and Health Sciences; Membrane filters; Messenger RNA; Metastases; Metastasis; MicroRNAs - genetics; miRNA; Myc protein; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; Neoplasm Invasiveness - prevention & control; Non-small cell lung carcinoma; Oncology; Respiratory diseases; Signal transduction; Signaling; Transcription factors; Wnt protein; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0172470

The present study was aimed to unravel the inhibitory mechanisms of curcumin for lung cancer metastasis via constructing a miRNA-transcription factor (TF)-target gene network. Differentially expressed miRNAs between human high-metastatic non-small cell lung cancer 95D cells treated with and without curcumin were identified using a TaqMan human miRNA array followed by real-time PCR, out of which, the top 6 miRNAs (miR-302b-3p, miR-335-5p, miR-338-3p, miR-34c-5p, miR-29c-3p and miR-34a-35p) with more verified target genes and TFs than other miRNAs as confirmed by a literature review were selected for further analysis. The miRecords database was utilized to predict the target genes of these 6 miRNAs, TFs of which were identified based on the TRANSFAC database. The findings of the above procedure were used to construct a miRNA-TF-target gene network, among which miR-34a-5p, miR-34c-5p and miR-302b-3p seemed to regulate CCND1, WNT1 and MYC to be involved in Wnt signaling pathway through the LEF1 transcription factor. Therefore, we suggest miR-34a-5p/miR-34c-5p/miR-302b-3p -LEF1-CCND1/WNT1/MYC axis may be a crucial mechanism in inhibition of lung cancer metastasis by curcumin.