Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal Polyps

• Published in: PloS one Vol. 11; no. 9; p. e0161013
• Main Authors: Xu, Jun, Han, Ruining, Kim, Dae Woo, Mo, Ji-Hun, Jin, Yongde, Rha, Ki-Sang, Kim, Yong Min
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.09.2016; Public Library of Science (PLoS)
• Subjects: Adult; Animal models; Animals; Asthma; Autoimmune diseases; Biology and Life Sciences; BLyS protein; Care and treatment; CD19 antigen; Cell activation; Chronic Disease; Complications and side effects; Cytokines; Dendritic cells; Enzyme-linked immunosorbent assay; Female; Flow cytometry; Gene expression; Health aspects; Health risks; Hospitals; Humans; Immunohistochemistry; Inflammation; Interferon; Interleukin 10; Interleukin 5; Interleukin-10 - physiology; Lymphocytes; Lymphocytes B; Male; Medicine; Medicine and Health Sciences; Mice; Mice, Inbred BALB C; Nasal polyps; Nasal Polyps - physiopathology; Nose; Otolaryngology; Pathogenesis; Pathogens; Patients; Polyps; Research and Analysis Methods; Rhinitis; Rhinitis - physiopathology; Rhinosinusitis; Risk factors; Sinusitis; Sinusitis - physiopathology; Surgery; Tissues; Western blotting; Young Adult; γ-Interferon
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0161013

Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine. The dysregulation of IL-10 is associated with an enhanced immunopathologic response to infection, as well as with an increased risk for developing numerous autoimmune diseases. In this study, we investigated IL-10 expression in chronic rhinosinusitis with nasal polyps (CRSwNP) and assessed the possible role of IL-10 in the pathogenesis of CRSwNP. Thirty-five patients with CRSwNP, 12 patients with chronic rhinosinusitis without NP (CRSsNP) and 10 control subjects were enrolled in this study. NP tissues and uncinated tissues (UT) were collected for analysis. Dispersed NP cells (DNPCs) were cultured in the presence or absence of IL-25 and IL-10, and a flow cytometric assay was performed to identify the constitutive cell populations of the DNPCs. Murine NP (n = 18) models were used for the in vivo experiments. Real-time PCR, immunohistochemistry, western blotting analysis and ELISA were performed to measure the expression levels of the selected inflammatory cytokines and inflammation-associated molecules. The mRNA expression levels of IL-10, IL-5, IL-17A, IL-25 and interferon gamma (IFN-γ) were significantly higher in the NP tissues than in the UT tissues. Strong positive correlations were observed between IL-10 and a variety of inflammatory cytokines (IL-5, IL-17A, IL-25, IFN-γ) and inflammation-associated molecules (B-cell activating factor; BAFF, CD19). Other than the IL-25 to IL-10 ratio, the expression ratios of the other measured inflammatory cytokines to IL-10 were significantly lower in the CRSwNP group than in the CRSsNP or control groups. Administrating IL-25 into the cultured DNPCs significantly increased the production of IL-10, but administrating IL-10 had no effect on the production of IL-25. Increased expression of IL-10, IL-10 related inflammatory cytokine, and IL-10 related B cell activation indicated that IL-10, a potent anti-inflammatory cytokine, has a pivotal role in the pathogenesis of CRSwNPs.