In Vitro Analysis of Predicted DNA-Binding Sites for the Stl Repressor of the Staphylococcus aureus SaPIBov1 Pathogenicity Island

• Published in: PloS one Vol. 11; no. 7; p. e0158793
• Main Authors: Papp-Kádár, Veronika, Szabó, Judit Eszter, Nyíri, Kinga, Vertessy, Beata G
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.07.2016; Public Library of Science (PLoS)
• Subjects: Bacterial proteins; Bacterial Proteins - genetics; Binding Sites; Biology and Life Sciences; Biotechnology; Complex formation; Deoxyribonucleic acid; DNA; DNA binding; DNA, Bacterial - analysis; dUTP pyrophosphatase; E coli; Escherichia coli; Food science; Genes; Genetic aspects; Genome, Bacterial; Genomes; Genomic Islands; Genomics; Health aspects; Laboratories; Medicine and Health Sciences; Metabolism; Nucleotide sequence; Oligonucleotides; Palindromes; Pathogenicity; Pathogens; Phages; Promoter Regions, Genetic; Properties; Proteins; Pyrophosphatases - genetics; Regulation; Repressor Proteins - genetics; Repressor Proteins - metabolism; Research and Analysis Methods; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus Phages - genetics; Virulence (Microbiology); Hungary
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0158793

The regulation model of the Staphylococcus aureus pathogenicity island SaPIbov1 transfer was recently reported. The repressor protein Stl obstructs the expression of SaPI proteins Str and Xis, latter which is responsible for mobilization initiation. Upon Φ11 phage infection of S. aureus. phage dUTPase activates the SaPI transfer via Stl-dUTPase complex formation. Our aim was to predict the binding sites for the Stl repressor within the S. aureus pathogenicity island DNA sequence. We found that Stl was capable to bind to three 23-mer oligonucleotides, two of those constituting sequence segments in the stl-str, while the other corresponding to sequence segment within the str-xis intergenic region. Within these oligonucleotides, mutational analysis revealed that the predicted binding site for the Stl protein exists as a palindromic segment in both intergenic locations. The palindromes are built as 6-mer repeat sequences involved in Stl binding. The 6-mer repeats are separated by a 5 oligonucleotides long, nonspecific sequence. Future examination of the interaction between Stl and its binding sites in vivo will provide a molecular explanation for the mechanisms of gene repression and gene activation exerted simultaneously by the Stl protein in regulating transfer of the SaPIbov1 pathogenicity island in S. aureus.