Characteristics of Aspergillus fumigatus in Association with Stenotrophomonas maltophilia in an In Vitro Model of Mixed Biofilm

• Published in: PloS one Vol. 11; no. 11; p. e0166325
• Main Authors: Melloul, Elise, Luiggi, Stéphanie, Anaïs, Leslie, Arné, Pascal, Costa, Jean-Marc, Fihman, Vincent, Briard, Benoit, Dannaoui, Eric, Guillot, Jacques, Decousser, Jean-Winoc, Beauvais, Anne, Botterel, Françoise
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.11.2016; Public Library of Science (PLoS)
• Subjects: Analysis; Antibiosis; Antibiotics; Antifungal agents; Antimicrobial agents; Aspergillus; Aspergillus fumigatus; Aspergillus fumigatus - genetics; Aspergillus fumigatus - physiology; Bacteria; Biofilms; Biofilms - growth & development; Biology and Life Sciences; Candida; Candida albicans; Cell walls; Chronic obstructive pulmonary disease; Conidia; Crystal structure; Cystic fibrosis; DNA, Bacterial - genetics; DNA, Fungal - genetics; Electron microscopy; Epithelium; Experimentation; Extracellular matrix; Fibrosis; Fluorescence; Fluorescence microscopy; Fungi; Fungicides; Glycerol; Green fluorescent protein; Health aspects; Hyphae; Immunocompromised hosts; In Vitro Techniques; Infection; Infections; Mathematical analysis; Medical research; Medicine and Health Sciences; Microbial Viability; Microorganisms; Microscopy; Microscopy, Electron, Scanning; Microscopy, Electron, Scanning Transmission; Models, Biological; Patients; Polystyrene; Polystyrene resins; Pseudomonas; Pseudomonas aeruginosa; Qualitative analysis; Real-Time Polymerase Chain Reaction; Research and Analysis Methods; Respiratory tract; Stenotrophomonas maltophilia; Stenotrophomonas maltophilia - genetics; Stenotrophomonas maltophilia - physiology; Transmission electron microscopy; France
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0166325

Biofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S. maltophilia and A. fumigatus by using analytical and quantitative approaches. An A. fumigatus strain (ATCC 13073) expressing a Green Fluorescent Protein (GFP) and an S. maltophilia strain (ATCC 13637) were used. Fungal and bacterial inocula (105 conidia/mL and 106 cells/mL, respectively) were simultaneously deposited to initiate the development of an in vitro mixed biofilm on polystyrene supports at 37°C for 24 h. The structure of the biofilm was analysed via qualitative microscopic techniques like scanning electron and transmission electron microscopy, and fluorescence microscopy, and by quantitative techniques including qPCR and crystal violet staining. Analytic methods revealed typical structures of biofilm with production of an extracellular matrix (ECM) enclosing fungal hyphae and bacteria. Quantitative methods showed a decrease of A. fumigatus growth and ECM production in the mixed biofilm with antibiosis effect of the bacteria on the fungi seen as abortive hyphae, limited hyphal growth, fewer conidia, and thicker fungal cell walls. For the first time, a mixed A. fumigatus-S. maltophilia biofilm was validated by various analytical and quantitative approaches and the bacterial antibiosis effect on the fungus was demonstrated. The mixed biofilm model is an interesting experimentation field to evaluate efficiency of antimicrobial agents and to analyse the interactions between the biofilm and the airways epithelium.