Enhanced Activities of Blood Thiamine Diphosphatase and Monophosphatase in Alzheimer's Disease

• Published in: PloS one Vol. 12; no. 1; p. e0167273
• Main Authors: Pan, Xiaoli, Sang, Shaoming, Fei, Guoqiang, Jin, Lirong, Liu, Huimin, Wang, Zhiliang, Wang, Hui, Zhong, Chunjiu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.01.2017; Public Library of Science (PLoS)
• Subjects: Acid Anhydride Hydrolases - blood; Aged; Aged, 80 and over; Aging; Alcohol; Alleles; Alzheimer Disease - blood; Alzheimer Disease - diagnosis; Alzheimer Disease - genetics; Alzheimer's disease; Alzheimers disease; Apolipoproteins E - genetics; Apolipoproteins E - metabolism; Biology and Life Sciences; Biomarkers; Blood; Blood Glucose; Blood levels; Care and treatment; Case-Control Studies; Chromatography; Chromatography, High Pressure Liquid; Cognitive ability; Collaboration; Dehydrogenases; Dementia; Dementia disorders; Dephosphorylation; Enzymes; Fasting; Female; Genotype; Glucose; Glucose metabolism; Health services; High performance liquid chromatography; Hospitals; Humans; Laboratories; Liquid chromatography; Male; Management; Medical care; Medicine and Health Sciences; Metabolism; Metabolites; Middle Aged; Neurobiology; Neurodegenerative diseases; Neurology; Neurosciences; Patients; Phosphatase; Phosphate esters; Phosphoric Monoester Hydrolases - blood; Phosphorylation; Physical Sciences; Reduction; Research and Analysis Methods; Science; Service centers; Thiamin Pyrophosphokinase - blood; Thiamine; Thiamine - blood; Thiamine diphosphate; Thiamine Monophosphate - blood; Thiamine pyrophosphate; Thyroid gland; Vitamin B
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0167273

Thiamine metabolites and activities of thiamine-dependent enzymes are impaired in Alzheimer's disease (AD). To clarify the mechanism for the reduction of thiamine diphosphate (TDP), an active form of thiamine and critical coenzyme of glucose metabolism, in AD. Forty-five AD patients clinically diagnosed and 38 age- and gender-matched control subjects without dementia were voluntarily recruited. The contents of blood TDP, thiamine monophosphate (TMP), and thiamine, as well as the activities of thiamine diphosphatase (TDPase), thiamine monophosphatase (TMPase), and thiamine pyrophosphokinase (TPK), were assayed by high performance liquid chromatography. Blood TDP contents of AD patients were significantly lower than those in control subjects (79.03 ± 23.24 vs. 127.60 ± 22.65 nmol/L, P<0.0001). Activities of TDPase and TMPase were significantly enhanced in AD patients than those in control subjects (TDPase: 1.24 ± 0.08 vs. 1.00 ± 0.04, P < 0.05; TMPase: 1.22 ± 0.04 vs. 1.00 ± 0.06, P < 0.01). TPK activity remained unchanged in AD as compared with that in control (0.93 ± 0.04 vs. 1.00 ± 0.04, P > 0.05). Blood TDP levels correlated negatively with TDPase activities (r = -0.2576, P = 0.0187) and positively with TPK activities (r = 0.2426, P = 0.0271) in all participants. Enhanced TDPase and TMPase activities may contribute to the reduction of TDP level in AD patients. The results imply that an imbalance of phosphorylation-dephosphorylation related to thiamine and glucose metabolism may be a potential target for AD prevention and therapy.