Insights into the Conformation of the Membrane Proximal Regions Critical to the Trimerization of the HIV-1 gp41 Ectodomain Bound to Dodecyl Phosphocholine Micelles

• Published in: PloS one Vol. 11; no. 8; p. e0160597
• Main Authors: Louis, John M, Baber, James L, Ghirlando, Rodolfo, Aniana, Annie, Bax, Ad, Roche, Julien
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.08.2016; Public Library of Science (PLoS)
• Subjects: Analysis; Antibodies; Biochemistry; Biology and Life Sciences; Cell fusion; Circular Dichroism; Clonal deletion; Crystallography; Diabetes; Electron Spin Resonance Spectroscopy; Epitopes; Glycoprotein gp41; HIV; HIV Envelope Protein gp41 - chemistry; HIV-1 - pathogenicity; Human immunodeficiency virus; Human immunodeficiency virus 1; Hydrogen ions; Kidney diseases; Laboratories; Lentivirus; Membrane proteins; Membranes; Membranes (Biology); Micelles; Models, Molecular; Molecular biology; Molecular Conformation; Monomers; Neural networks; Peptides; pH effects; Phosphocholine; Phosphorylcholine - analogs & derivatives; Phosphorylcholine - chemistry; Physical Sciences; Physics; Protein Domains; Proteins; Research and Analysis Methods; Residues; Sedimentation; Sedimentation & deposition; Sequence Analysis, Protein; Spectroscopy; Studies; Virus Attachment; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160597

The transitioning of the ectodomain of gp41 from a pre-hairpin to a six-helix bundle conformation is a crucial aspect of virus-cell fusion. To gain insight into the intermediary steps of the fusion process we have studied the pH and dodecyl phosphocholine (DPC) micelle dependent trimer association of gp41 by systematic deletion analysis of an optimized construct termed 17-172 (residues 528 to 683 of Env) that spans the fusion peptide proximal region (FPPR) to the membrane proximal external region (MPER) of gp41, by sedimentation velocity and double electron-electron resonance (DEER) EPR spectroscopy. Trimerization at pH 7 requires the presence of both the FPPR and MPER regions. However, at pH 4, the protein completely dissociates to monomers. DEER measurements reveal a partial fraying of the C-terminal MPER residues in the 17-172 trimer while the other regions, including the FPPR, remain compact. In accordance, truncating nine C-terminal MPER residues (675-683) in the 17-172 construct does not shift the trimer-monomer equilibrium significantly. Thus, in the context of the gp41 ectodomain spanning residues 17-172, trimerization is clearly dependent on FPPR and MPER regions even when the terminal residues of MPER unravel. The antibody Z13e1, which spans both the 2F5 and 4E10 epitopes in MPER, binds to 17-172 with a Kd of 1 ± 0.12 μM. Accordingly, individual antibodies 2F5 and 4E10 also recognize the 17-172 trimer/DPC complex. We propose that binding of the C-terminal residues of MPER to the surface of the DPC micelles models a correct positioning of the trimeric transmembrane domain anchored in the viral membrane.