Research on Tobacco Transformation of Vacuolar H＋-ATPase Subunit c Gene from Iris lacteal

Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacco, and then treated the transgenic tobacco with 0.7% NaC1 for 7 days. Detected the MDA content, the...

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Published inThe journal of Northeast Agricultural University Vol. 19; no. 3; pp. 43 - 48
Main Authors Duo, Wu, Ai-min, Zhou, Sheng-feng, Shi, Shan-shan, Li, Jin-gang, Wang
Format Journal Article
LanguageEnglish
Published 01.09.2012
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Summary:Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacco, and then treated the transgenic tobacco with 0.7% NaC1 for 7 days. Detected the MDA content, the SOD activity and the POD activity. Moreover, the transgenic plants displayed obviously better growth than the normal plants under salt stress. These results suggested that VHA-c could confer the stress tolerance through enhancing the POD and the SOD activities, and by protecting membranes from damage by decreasing lipid peroxidation under salt stress.
Bibliography:Vacuolar ATPase is the key enzyme in the aspect of plant stress, which contains 13 subunits. In this study, VHA-c which was cloned from the salt-tolerant plants Iris lacteal was transformed into tobacco, and then treated the transgenic tobacco with 0.7% NaC1 for 7 days. Detected the MDA content, the SOD activity and the POD activity. Moreover, the transgenic plants displayed obviously better growth than the normal plants under salt stress. These results suggested that VHA-c could confer the stress tolerance through enhancing the POD and the SOD activities, and by protecting membranes from damage by decreasing lipid peroxidation under salt stress.
VHA-c, Nicotiana tabacum L.cv., salt stress
23-1392/S
ISSN:1006-8104
DOI:10.1016/S1006-8104(13)60021-5