Comparison of serum and plasma as a source of blood extracellular vesicles: Increased levels of platelet-derived particles in serum extracellular vesicle fractions alter content profiles from plasma extracellular vesicle fractions

Extracellular vesicles (EVs) have attracted much attention as potential diagnostic biomarkers for human diseases. Although both plasma and serum are utilized as a source of blood EVs, it remains unclear whether, how and to what extent the choice of plasma and serum affects the experimental results....

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Published inPloS one Vol. 17; no. 6; p. e0270634
Main Authors Zhang, Xiaoman, Takeuchi, Toshihide, Takeda, Akiko, Mochizuki, Hideki, Nagai, Yoshitaka
Format Journal Article
LanguageEnglish
Published San Francisco Public Library of Science 24.06.2022
Public Library of Science (PLoS)
Subjects
Alzheimer's disease
Analysis
Anticoagulants
Apolipoproteins
Biology and Life Sciences
Biomarkers
Blood
Blood plasma
Blood platelets
Experiments
Extracellular vesicles
Literature reviews
Medicine and Health Sciences
Nanoparticles
Ostomy
Phlebotomy
Plasma
Platelets
Proteins
Proteomes
Proteomics
Research and Analysis Methods
Vesicles
Japan
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Summary:Extracellular vesicles (EVs) have attracted much attention as potential diagnostic biomarkers for human diseases. Although both plasma and serum are utilized as a source of blood EVs, it remains unclear whether, how and to what extent the choice of plasma and serum affects the experimental results. To address this issue, in this study, we performed comprehensive characterization of EV fractions derived from plasma and serum, and investigated the differences between these blood EVs. We demonstrated by nanoparticle tracking analysis that EV fractions derived from serum contain more particles than those from plasma of mice. Proteomic analysis demonstrated that platelet-associated proteins are selectively enriched in serum EV fractions from both mice and humans. A literature review of proteomic data of human blood EVs reported by other groups further confirmed that selective enrichment of platelet-associated proteins is commonly observed in serum EVs, and confers different proteome profiles to plasma EVs. Our data provide experimental evidence that EV fractions derived from serum generally contain additional EVs that are released from platelets, which may qualitatively and quantitatively alter EV profiles when using serum as a source of blood EVs.
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Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: [XZ, TT, AT, and YN belonged to Department of Neurotherapeutics, Osaka University Graduate School of Medicine, an endowment department that was supported by Nihon Medi-Physics Co., AbbVie GK., Otsuka Pharm Co., Kyowakai Med. Co., Fujiikai Med. Co., Yukioka Hosp., Osaka Gyoumeikan Hosp., Kyorin Co., and Tokuyukai Med. Co. Other authors declare no conflict of interest.] This does not alter our adherence to PLOS ONE policies on sharing data and materials.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0270634