High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons

The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary el...

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Published inPloS one Vol. 6; no. 1; p. e16148
Main Authors Deshmane, Satish L, Mukerjee, Ruma, Fan, Shongshan, Sawaya, Bassel E
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 07.01.2011
Public Library of Science (PLoS)
Subjects
Acquired immune deficiency syndrome
AIDS
AIDS Dementia Complex - etiology
Apoptosis
Biology
Capillary electrophoresis
Cell Communication
Cell Line
Cognition
Cyclin-dependent kinases
Electrophoresis
Electrophoresis, Capillary - methods
Gene Products, tat - analysis
Gene Products, tat - blood
Genomes
Health aspects
HIV
HIV infections
HIV patients
HIV-1 - chemistry
Human immunodeficiency virus
Human immunodeficiency virus 1
Humans
Infection
Infections
Laboratories
Medicine
Neuroblastoma
Neurodegeneration
Neurons
Neurons - chemistry
Neurons - virology
Neurosciences
Neurotoxicity
Pathogenesis
Pneumonia
Proteins
RNA polymerase
Studies
Tat protein
United States > US
Pennsylvania
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Summary:The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders.
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Conceived and designed the experiments: BES. Performed the experiments: SLD RM SF. Analyzed the data: BES. Contributed reagents/materials/analysis tools: SLD RM SF. Wrote the paper: BES.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0016148